A comparative study on in vitro and in vivo antioxidant activities of aqueous extract of Desmodium gangeticum (Leguminosae) root
DOI:
https://doi.org/10.22377/ijgp.v3i4.110Abstract
The present study was designed to investigate the in vitro and in vivo free radical scavenging effect of aqueous extract of Desmodium gangeticum (DG) root in different antioxidant models and experimentally induced ischemic reperfusion in an isolated rat heart. The rats were divided into three groups namely control, reperfusion control, and drug treated. For in vivo study, ischemic reperfusion injury (IRI) was induced by 30 minutes ischemia followed by reperfusion of Krebs-Henseleit buffer for 15 minutes or 30 minutes or 45 minutes. Oral administration of DG extract (50 and 100 mg/kg once daily for 30 days) was performed in rats of drug group and anincrease of enzymatic activity of SOD, catalase and GPx along with an inhibition of lipid per-oxidations were observed. However, a significant rise in lipid peroxidation and reduction in myocardial SOD, catalase, and GPx were observed during IRI. The inhibitory
concentrations (IC50) in DPPH, superoxide scavenging activity, hydroxide scavenging activity, nitric oxide scavenging activity and lipid peroxidation were found to be 51.3, 24.6, 52.7, 53.2 and 415 mg/mL respectively. The presence of cactine, an alkaloid used to treat irregular heartbeat, angina pectoris and cardiac neuralgia was reported in DG through GS-MS analysis. The above results suggest that the aqueous extract of DG root exhibit potential free radical scavenging effect that can reduce the oxidative stress exhibited by IRI.
Key words: Desmodium gangeticum, GS-MS, in vitro antioxidants, myocardial ischemia reperfusion
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References
Rice Evan CA. Formation of free radicals and mechanisms of action
in normal biochemical processes and pathological states. In: Free
radical damage and its control. Elsevier science; 1994. p. 131-53.
Gursahan HI. Schaefer S. Acidification reduces mitochondrial
calcium uptake in rat cardiac mitochondria. Am J Physiol Heart
Circ Physiol 2004;287:H2659-65.
Jayaprakasha GK, Selvi T, Sakariah KK. Antibacterial and
antioxidant activities of grape (Vitis vinifera) seed extracts. Food
Res Intern 2003;36:117-22.
Sun J, Tan BK, Hunag SH, Whiteman M, Zhu YZ. Effects of natural
products on ischemic heart diseases and cardiovascular system.
Acta pharmacologica Sinica 2002;23:1142-51.
Hong CY, Huang SS, Tsai SK. Magnolol reduces infarct size and
suppresses ventricular arrhythmia in rats subjected to coronary
ligation. Clin Exp Pharmacol Physiol 1996;23:660-4.
Tsai SK, Huang SS, Hong CY. Myocardial protective effect of
honokiol: An active component in Magnolia officinalis. Planta
medica 1996;62:503-6.
Kirthikar KR, Basu BD. In: Indian Medicinal Plants. Vol.1.
Allahabad, India; Lalith Mohan Basu; 1975. p. 758-60.
Govindarajan R, Vijayakumar M, Rao CV, Shirwaikar A, Kumar S,
Rawat AK, et als. Antiinflammatory and antioxidant activities of
Desmodium gangeticum fractions in carrageenan‑induced inflamed
rats. Phytother Res 2007;21:975-9.
Mishra PK, Singh N, Ahmad G, Dube A, Maurya R. Glycolipids
and other constituents from Desmodium gangeticum with
antileishmanial and immunomodulatory activities. Bioorg Med
Chem Lett 2005;15:4543-6.
Johnson D, Lardy H. Isolation of liver or kidney mitochondria.
In: Estrabrook RW, editor. Methods in enzymology. Academic
Press; 1967. p. 94-6.
Okhawa H, Oohishi N, Yagi K. Assay for lipid peroxides in animal
tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
Marklund SL. Pyragallol autooxidation. In: Greenwald RA, editor.
Handbook of methods of oxygen radical research. Boca Raton,
CRC Press; 1985. p. 243-7.
Geller BL, Winge DR. A method for distinguishing CuZn SOD and
Mn containing superoxide dismutases. Anal Biochem 1983;128:86‑92.
Aebi HE. Catalase. In: Bergmeyer HU, editor. Methods of enzymatic
analysis. 3rd ed. VCH Verlagsgesellschatt mbH; 1985. p. 273-8.
Wendel A. Glutathione peroxidase. In: Methods in Enzymology.
Vol. 77. 1981. p. 325-33.
Lowry OH, Rosenbrough NT, Farr AL. Protein measurements with
the Folin phenol reagent. J Biol Chem 1951;193:265-75.
Misra HP, Fridovich I. The univalent reduction of oxygen by
reduced flavins and quinines. J Biol Chem 1972;247:188-92.
Halliwell B, Gutteridge JMC, Aruoma OI. The deoxyribose method:
A simple “test tube†assay for determination of rate constants for
reactions of hydroxyl radicals. Anal Biochem 1987;165:215-9.
Blois MS. Antioxidant determination by the use of a stable free
radical. Nature 1958;181:1199-200.
Marcocci L, Maguire JJ, Droy - Lefaix MT, Packer L. The nitric
oxide-scavenging properties of Ginkgo biloba extract EGb 761.
Biochem Biophys Res Commun 1994;201:748-55.
Singh N, Mishra PK, Kapil A, Arya KR, Maurya R, Dube A.
Efficacy of Desmodium gangeticum extract and its fractions
against experimental visceral leishmaniasis. J Ethnopharmacol
;98:83‑8.
Zaugg M, Lucchinetti E, Garcia C, Pasch T, Spahn DR, Schaub MC.
Anaesthetics and cardiac preconditioning. Part II. Clinical
implications. Br J Anaesth 2003;91:566-76.
Braugghler JM, Duncan CA, Chase LR. The involvement of iron in
lipid peroxidation: Importance of ferrous to ferric ratio in initiation.
J Biol Chem 1986;261:10282-7.
Rice-Evans CA, Sampson J, Bramley PM, Holloway DE. Why do
we expect carotenoids to be antioxidants in vivo. Free Radic Res
;26:381-98.
Kloner RA, Jennings RB. Consequences of brief ischemia: Stunning,
preconditioning, and their clinical implications. Circulation
;104:3158-67
