The research of clonal micropropagation efficiency of Schisandra chinensis under the influence of low-intensity coherent radiation

Abstract

Aim: The present study reveals the results of an investigation on the effect of low-intensity coherent radiation (LCR) on the efficacy of clonal micropropagation of medicinal plant - Schisandra chinensis (Turcz.) Baill. Experimental: Cultivation of plants in vitro, a mineral base of the nutrient medium was used with the addition of 30 g/l sucrose, 100 mg/l mesoinositol, 250 mg/l casein hydrolyzate, 8 g/l agar and vitamin complex of Murashige-Skoog. During microcuttings, rooting macrosalts and sucrose concentration reduced to half; indole butyric acid at a concentration kept at 1.0 mg/l. Sterile microcuttings were treated with radiation from a helium-neon laser, GN-40 (wavelength 632.8 nm, power density 2 W/m2), and semiconductor HLDPM12-655-10HJ (wavelength 655 nm, power density 2 W/m2) at various exposures (30, 60, 120, 240, 480, 960 s) on 3-4 days after their planting into the propagation medium or rooting directly in the culture bottles. Result and Discussion: It has been established, that the use of LCR at the stage of rhizogenesis significantly stimulates the process of microcuttings rooting in vitro and promotes an increase in the number and length of roots and growth of shoots. The selection of in vitro conditions and irradiation treatment of the obtained S. chinensis microshoots by helium-neon laser, during the propagation stage, allow to increase the number of viable explants of this culture from 60.0% ± 6.3% in the control sample, up to 90.9% ± 3.5% in the test sample with 120 s exposure of irradiation. With this exposure, the share of microshoots with a net reproduction more than 1, increased from 44.4% to 75.5%. Conclusion: Due to low rates of reproduction and rooting, a slow growth rate of shoots and roots, latent bacterial infections, significantly reduce the method clonal micropropagation for S. chinensis. Laser coherent radiation stimulates the process of microcuttings rooting in vitro at the stage of rhysogenesis of S. chinensis (Turcz.) Baill leads to increase in number and length of root and shoot system.