Characteristics of 2-(tetrazol[1,5-c]quinazolin-5-ylthio) acetate effect on the level of adenine nucleotides in intense physical activity

Abstract

Introduction: We investigated changes in the energy state of the skeletal muscle cells in rats under physical load (15 days). Materials and Methods: The experiments were performed on nonlinear white rats. On the 15th day of the experiment, the rats were loaded with running at a speed of 42 m/min and slope angle 10°. The test substance sodium 2-(tetrazol[1,5-c]quinazolin-5-ylthio)acetate (compound КВ-28) at a dose of 1.7 mg/kg and reference compound 2-ethylthiobenzimidazole hydrobromide (32.0 mg/kg) were administered to animals intraperitoneally 60 min before treadmill training. Concentration of adenine nucleotides (ATP, ADP, and AMP) was determined in skeletal muscles by thin layer chromatography test. Results and Discussion: It was first found that a course of intraperitoneal injection of sodium 2-(tetrazol[1,5-c]quinazolin-5-ylthio)acetate (compound KB-28) contributed to 88.5% (P < 0.05) growth of physical endurance. We proved, that unlike the trained control, administration of compound KB-28 prevented imbalance in the system of adenine nucleotides. It was indicated by a significantly important increase of the level of ATP by 54.8% while parallel reducing concentration of ADP and AMP by 16.8 and 43.4%, respectively. Conclusions: Restoration of balance in the adenine nucleotides system may be one of the components of sodium 2-(tetrazol[1,5-c]quinazolin-5-ylthio)acetate (compound KB-28) actoprotective effect.