Preventive effect of Zanthoxylum armatum fruit on mitochondrial lipids alteration in isoproterenol-induced myocardial infarcted rats

Abstract

Background: Mitochondrial lipids are essential for the mitochondrial architecture, the activity of respiratory proteins, and the transport of proteins into the mitochondria. Aim: In this study, the preventive role of Zanthoxylum armatum fruit on mitochondrial lipids alteration induced by isoproterenol hydrochloride (ISO) was evaluated in myocardial infarcted (MI) rats. Methods: Oral pre-treatment with hydroethanolic extract of Z. armatum fruit to ISO-induced rats was done for 30 days followed by the isolation of mitochondria from the heart tissue. The lipids such as total cholesterol (TC), phospholipids (PL), triglycerides (TG), and free fatty acids (FFA) in the heart mitochondria and lipid hydroperoxides and thiobarbituric acid reactive substances (TBARS) in the heart tissue were evaluated. Results: ISO-induced MI rats showed a significantly (P < 0.05) increased mitochondrial TC, TG, and FFA with a subsequent decrease in PL. Significantly increased levels of lipid hydroperoxides and TBARS were observed in the heart tissue of ISO-treated rats. Oral pre-treatment with hydroethanolic extract of Z. armatum fruit to ISO-induced rats significantly (P < 0.05) reverted these alterations near to normal status. Conclusion: Pre-treatment with Z. armatum fruit favorably restored the biochemical alterations demonstrating that Z. armatum fruit has a significant protective effect on cardiac mitochondrial function against ISO-induced MI in rats.