Development of methods for the chromatographic identification of active pharmaceutical ingredient from group of angiotensin-converting enzyme inhibitors in pharmaceuticals

Abstract

Introduction: Thin-layer chromatography (TLC) is a chromatography used to separate non-volatile mixtures. TLC can be used for monitoring the progress of a reaction, identification compounds present in a given mixture, and determination of purity of an active pharmaceutical ingredient (API). Analysis of API from group of angiotensin-converting enzyme inhibitors is described in pharmacopeia, but the aim of our researchers was to improve to more rapid, simple, and less expensive methods. TLC analysis of enalapril, captopril, fosinopril, and perindopril in pharmaceuticals and for using this method of analysis in future for the development of bioanalytical methods. Materials and Methods: The present study is assessed system solvents of enalapril, captopril, fosinopril, and perindopril for TLC. Results: We have established that the most perfect Rf observed using mobile phases: Ammonia (25%) - propanol (30:70) for enalapril, chloroform - methanol (9:1) for captopril, ammonia (25%) - propanol (30:70) for perindopril, and n-butanol - methanol (3:2) for fosinopril. However, those mobile phases are the most expressive. We have explored the validation characteristics - specificity and suitability of the chromatographic system according to SPU. Conclusion: We have developed chromatographic methods of identification of enalapril, captopril, fosinopril, and perindopril in pharmaceuticals. The developed methods are rapid, economical, simple, and applicable to the analysis of pharmaceutical dosage forms. These methods can also give excellent results and can be employed for the routine analysis. Prospects for future research will be aimed at developing of bioanalytical methods of determination of enalapril, captopril, fosinopril, perindopril in medicines, and for analysis of their metabolites.