Chemical composition and antimicrobial activity of the volatile oil of Eucalyptus sargentii Maiden cultivated in central Iran
DOI:
https://doi.org/10.22377/ijgp.v4i3.141Abstract
Eighteen components were identified from the leaves oil of Eucalyptus sargentii Maiden, which were collected in the autumn from Kashan (Isfahan Province, Iran) by GC and GC-MS, representing 98.0% of total oil. The main constituents of the oil were 1,8-cineole (55.48 %), α-pinene (20.95 %), aromadendrene (6.45 %), and trans-pinocarveol (5.92%). In vitro antimicrobial activity of the oil of E. sargentii was tested against three Gram-positive (Bacillus subtilis, Staphylococcus aureus, and Staphylococcus epidermidis), five Gram-negative (Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus vulgaris, Shigella dysenteriae) bacteria,and two fungi (Aspergillus niger and Candida albicans). The results of the bioassay showed that the oil exhibited moderate to high antimicrobial activity.
Key words: 1,8-cineole, antimicrobial activity, Eucalyptus sargentii Maiden, volatile oil, α-pinene
Downloads
References
Reynolds JEF. Martindale-the Extra Pharmacopeia. 31st ed. London:
Royal Pharmaceutical Society of Great Britain; 1996.
Lis-Balchin M, Deans SG. Bioactivity of selected plant essential oils
against Listeria monocytogenes. J Appl Bacteriol 1997;82:759-62.
Bhattacharjee SK, Handbook of aromatic plants. Jaipur, India:
Pointer Publishers; 2005. p. 190-2.
Silva J, Abebe W, Sousa SM, Duarte VG, Machado MI, Matos
FJ. Analgesic and anti-inflammatory effects of essential oils of
Eucalyptus. J Ethnopharmacol 2003;89:277-83.
Williams LR, Stockley JK, Yan W, Home VN. Essential oils with
high antimicrobial activity for therapeutic use. Int J Aromather
;8:30-40.
Boland DJ, Broppy JJ, House AP. Eucalyptus leaf oils: Use,
chemistry, distillation. Melbourne: Inkata Press; 1991.
Sacchetti G, Maietti S, Muzzoli M, Scaglianti M, Manfredini S,
Radice M, et al. Comparative evaluation of 11 essential oils of
different origin as functional antioxidants, antiradicals and
antimicrobials in foods. Food Chem 2005;91:621-32.
Delaquis PG, Stanich K, Girard B, Mazza G. Antimicrobial activity
of individual and mixed fractions of dill, cilantro, coriander and
eucalyptus essential oils. Int J Food Microbiol 2002;74:101-9.
Nishimura H, Calvin M. Essential oil of eucalyptus globules in
California. J Agric Food Chem 1979;27:432-5.
Magiatis P, Skaltsounis AL, Chinou I, Haroutounian AS. Chemical
composition and in-vitro antimicrobial activity of the essential oils
of three Greek Achillea species. Z Naturforsch C 2002;57:287-90.
Obeng Ofori D, Reichmuth CH, Bekele J, Hassanali A. Biological
activity of 1,8 cineole: A major component of essential oil of
Ocimum kenyense (Ayobangira) against stored product beetles. J
Appl Ent-Zeit Angew Ent 1997;121:237-43.
Sefidkon F, Assareh MH, Abravesh Z, Mirza M. Chemical
composition of the essential oils of five cultivated Eucalyptus
species in Iran: E. intertexta, E. platypus, E. leucoxylon, E.
sargentii and E. camaldulensis. J Essent Oil Bearing Plants
;9:245-50.
Bignell CM, Dunlop PJ, Brophy JJ Jackson JF. Volatile Leaf Oils
of some South-western and Southern Australian Species of
the Genus Eucalyptus: Part XI: Subgenus Symphyomyrtus. A -
Section Bisectaria. (a) Series Occidentales, (b) Unpublished series
Annulatae, (c) Series Micromembranae, (d) Series Obliquae, (e)
Series Dundasianae, (f) Series Cooperianae, (g) Series Halophilae,
(h) Series Salmonophloiae, and (i) Series Pubescentes: B -Section
Dumaria, (a) Series Merrickianae. Flavour Frag J 1996;11:10712.
Jennings W, Shibamoto T. Qualitative analysis of flavour and
fragrance volatiles by glass capillary gas chromatography. New
York: Academic Press; 1980.
Adams RP. Identification of essential oil components by gas
chromatography/mass spectroscopy. 4th ed. Carol Stream, IL:
Allured Publishing; 2007.
Wayne PA. Performance Standards for Antimicrobial Susceptibility
Testing, 9th International Supplement, M100-S9. National
Committee for Clinical Laboratory Standards (NCCLS); 1999.
Daferera DJ, Ziogas BN, Polissiou MG. GC-MS analysis of essential
oils from some Greek aromatic plants and their fungitoxicity on
Penicillium digitatum. J Agric Food Chem 2000;48:2576-81.
Panizzi L, Flamini G, Cioni PL, Morelli I. Composition and
antimicrobial properties of essential oils of four Mediterranean
Lamiaceae. J Ethnopharmacol 1993;39:167-70.
Burt S. Essential oils: Their antibacterial properties and potential
applications in foods: A review. Int J Food Microbiol 2004;94:223-53.
