Extraction of Ashwagandha by conventional extraction methods and evaluation of its anti-stress activity
DOI:
https://doi.org/10.22377/ijgp.v4i3.143Abstract
The present study was conducted to compare the yield and the antistress activity of Ashwagandha (Withania somnifera) extracts,using two extraction methods: hot continuous percolation and maceration. Various parameters like temperature, extraction time (10 hours) solvents (water, alcohol, hydroalcohol) and drug-solvent ratios (1:6, 1:8, 1:10) were fixed. The highest yield was found to be 16.96% w/w by maceration process using water (1:8). The activity of different extracts was done by Plus-Maze model using alprazolam as the standard drug. Significant result was found in water extract and hydroalcoholic (1:8) extract prepared by maceration method and also in hydro-alcoholic extract (1:8) prepared by soxhlet process.
Key words: Alprazolam, anti-stress activity, elevated plus maze, extraction, withania somnifera
Downloads
References
Jain NK. Sharma SN. A textbook of profesional pharmacy. 4th ed.
Delhi: Vallabh Prakashan; 2003. p. 296-8.
Mukherjee PK. Quality control of herbal drugs. Pharmaceutical
Publishers; 2003. p. 571-3.
Tripathi KD. Essentials of medicinal pharmacology. 5th ed. New
Delhi: 2003. p. 405.
Andallu B, Radhika B. Hypoglycemic diuretic and
hypocholesterolemic effect of winter cherry (Withania somnifera
Dunal) root. Indian J Exp Biol 2000;38:607-9.
Bhatia P, Rattan SIS, Cavallius J, Clark BFC. Withania somnifera
(Ashwagandha) a so-called rejuvenator inhibits growth and
macromolecular synthesis of human cells. Med Sci Res 1987;15:515-6.
Kokate CK, Analytical pharmacognosy. 15th ed. Pune: Nirali
Prakashan; 2000. p. 48.
Khandelwal KR. Practical pharmacognosy: Techniques and
experiment. 16th ed. Nirali Prakashan; 2006. p. 149-54.
Trease and Evans, Pharmacognosy. 15th ed. W.B. Saunders; 2002. p. 46.
OECD Guidelines for testing of chemicals Acute Toxicity-Fixed
Dose Procedure 2004. p. 420.
Kulkarni SK. Handbook of experimental pharmacology. 2nd ed.
New Dehli: Vallabh Prakashan; 1993. p. 5.
