Aqueous extract of Salvia officinalis and Ruta graveolens: Potential source of reactive nitrogen species
DOI:
https://doi.org/10.22377/ijgp.v4i3.145Abstract
There are scarce evidences about the effects of herbs on nitrogen species that induced nitrosative stress. We here investigated the effect of simple water distilled extract of dry leaves salvia officinalis (sage) and Ruta graveolens (Rue) on the nitric oxide (NO) – peroxynitrite (ONOO-) cycle biochemistry in vitro experiments. Aqueous extract of sage or rue (1%) were prepared by simple distillation and scanned by UV-visible spectrophotometer. Their effects were studied on the synthesized ONOO- as well as their ability to generate ONOO. It is ability to donate NO or to scavange released NO by sodium nitroprusside (10 mM) also investigated. UV-visible scan of sage extract revealed the presence of peaks at λ195 and 348.5 while that of rue extract at λ 200, λ242, λ291.5. Both extracts notgenerate ONOO- radical in form of nitrophenols. Rue extract increased the yield of prepared ONOO- by more than five times. Rue extract donated NO and improved the release NO from sodium nitroprusside while sage extract only improved the release of NO released by sodium nitroprusside. We conclude that simple distilled - aqueous extract of rue and sage extracts improved nitric oxide bioavailability that may be helpful in coronary artery disease with nitrate tolerance.
Key words: Nitric oxide, Peroxynitrite, Ruta graveolens, Salvia officinalis
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Lima CF, Valentao PC, Andrade PB, Seabra RM, Fernandes-
Ferreira M, Pereira-Wilson C. Water and methanolic extracts
of Salvia officinalis protect HepG2 cells from t-BHP induced
oxidative damage. Chem Biol Interact 2007;167:107-15.
Reuter J, Jocher A, Hornstein S, Mönting JS, Schempp CM. Sage
extract rich in phenolic diterpenes inhibits ultraviolet-induced
erythema in vivo. Planta Med 2007;73:1190-1.
Bozin B, Mimica-Dukic N, Samojlik I , Jovin E. Antimicrobial
and antioxidant properties of rosemary and sage (Rosmarinus
officinalis L. and Salvia officinalis L., Lamiaceae) essential oils. J
Agric Food Chem 2007; 55:7879-85.
Oniga I, Pârvu AE, Toiu A, Benedec D. Effects of Salvia officinalis
L. extract on experimental acute inflammation. Rev Med Chir Soc
Med Nat Iasi 2007;111:290-4.
Preethi KC, Kuttan G, Kuttan R. Anti-tumour activity of Ruta
graveolens extract. Asian Pac J Cancer Prev 2006;7:439-43.
Raghav SK, Gupta B, Agrawal C, Goswami K, Das HR. Antiinflammatory
effect of Ruta graveolens L. in murine macrophage
cells. J Ethnopharmacol 2006;104:234-9.
Whiteman M, Halliwell B. Protection against peroxynitritedependent
tyrosine nitration and alpha 1-antiproteinase
inactivation by ascorbic acid: A comparison with other biological
antioxidants. Free Radic Res 1996;25:275-83.
Beckman JS, Ischiropoulos H, Zhu L, van der Woerd M, Smith C,
Chen J, et al. Kinetics of superoxide dismutase and iron –catalyzed
nitration of phenolics by peroxynitrite. Arch Biochem Biophys
;298:438-45.
VanUffelen BE, Van Der Zee J, De Koster BM, Vanstereninck J,
Elferink JG. Intracellular but not extracellular conversion of
nitroxyl anion into nitric oxide leads to stimulation of human
neutrophil migration. Biochem J 1998;330:719-22.
Newaz MA, Yousefipour Z, Nawal N, Adeeb N. Nitric oxide
synthase activity in blood vessels of spontaneously hypertensive
rats: Antioxidant protection by gamma-tocotrienol. J Physiol
Pharmacol 2003;54:319-27.
Sundararajan R, Haja NA, Venkatesan K, Mukherjee K, Saha
BP, Bandyopadhyay A, et al. Cytisus scoparius link: A natural
antioxidant. BMC Complement Altern Med 2006;6:8.
Kim JY, Kim HS, Kang HS, Choi JS, Yokozawa T, Chung HY.
Antioxidant potential of dimethyl lithospermate isolated from
Salvia miltiorrhiza (red sage) against peroxynitrite. J Med Food
;11:21-8.
Tanaka H, Hattori H, Tanaka T, Sakai E, Tanaka N, Kulkarni A, et al.
A new Erythrina alkaloid from Erythrina herbacea. Nat Med
(Tokyo) 2008;62:228-31.
Kapadia MR, Chow LW, Tsihlis ND, Ahanchi SS, Eng JW, Murar J,
et al. Nitric oxide and nanotechnology: A novel approach to
inhibit neointimal hyperplasia. J Vasc Surg 2008;47:173-82.
Pathak S, Multani AS, Banerji P, Banerji P. Ruta 6 selectively
induces cell death in brain cancer cells but proliferation in normal
peripheral blood lymphocytes: A novel treatment for human
brain cancer. Int J Oncol 2003;23:975-82.
Liu P, Ge YC, Ma TS, Ren HJ, Xu YJ, Xu DM. Effects of the
extracts from decoction for resuscitation and its component
herbs on PGI2, TXA2 and NO release from rat vascular
endothelial cells under hypoxia in vitro. Zhongguo Zhong Yao
Za Zhi 2004;29:988-92.
Zhao HW, Li XY. Ginkgolide A, B, and huperzine A inhibit
nitric oxide-induced neurotoxicity. Int Immunopharmacol
;2:1551-6.
Al-Nimer and Ali: Rue, sage, nitrogen species
Source of Support: Nil, Conflict of Interest: None declared.
Yin XY, Jiang JM, Liu JY, Zhu JR. Effects of endogenous nitric
oxide induced by 5-fluorouracil and L-Arg on liver carcinoma
in nude mice. World J Gastroenterol 2007;13:6249-53.
