Evaluation of acute toxicity of Tribhuvana-Mishrana in albino rats

Abstract

Background: Tribhuvana- Mishrana (TM) is a complex herbo-mineral preparation used as an antipyretic agent. It is an effective remedy for Sannipata (~vitiation of all doshas/severe condition of any disease), Sarva Jvara (~all types of fever), and Pratishyaya (~coryza). Objective: The present work was conducted to establish the safety aspect of the use of TM. Study and Design: TM is the combination of three drugs - Tribhuvan Kirti Rasa (TKR), Godanti Bhasma (GB), and Sudarshan Ghanvati (SGV). For the preparation of TM, TKR, GB and SGV were prepared separately by an assortment of Ayurvedic procedures such as Shodhana (~purification), Marana (~calcination), and Bhavana (~lavigation). Then, fine powder of above three mentioned drugs was mixed homogenously as per the reference of Ayurveda Formulary of India. The resultant product was subjected to acute toxicity study. Materials and Methods: Acute toxicity study was conducted in Wister strain of albino rats as per the OECD guidelines 423. Criteria of assessment included behavior changes, hematological changes, and histopathological changes. Histopathological studies of different organs include brain, liver, kidney, and spleen were also conducted to observe pathological changes if any. Results: In acute toxicity study TM was found to be safe. Rats did not showed any signs of toxicity. All hematological parameters, namely, hemoglobin%, total leukocyte count, differential leukocyte count, and total count were found to be within normal limits. Histopathological study of different organs revealed normal cytoarchitecture