New derivative spectrophotometric methods for the determination of thiocolchicoside – A semisynthetic derivative of colchicoside

Abstract

Introduction: Thiocolchicoside is a semisynthetic derivative of colchicoside. It is clinically as anti-inflammatory, analgesic, and muscle relaxant. New spectrophotometric techniques have been proposed for the determination of thiocolchicoside in hydrochloric acid, sodium acetate buffer, and phosphate buffer (pH 7.0). Materials and Methods: Shimadzu double beam UV-VIS spectrophotometer (UV-1800 Model) was used for the present study. Zero-order and first derivative spectrophotometric techniques have been developed for the determination of thiocolchicoside in hydrochloric acid, sodium acetate buffer, and phosphate buffer (pH 7.0). Results and Discussion: Thiocolchicoside has shown absorption maxima at 259 nm in all the methods, and linearity was observed 1.0–70, 0.1–2, and 0.1–6 μg/ml in hydrochloric acid, sodium acetate buffer, and phosphate buffer, respectively, and all the methods were validated as per ICH guidelines. Conclusions: These methods are simple and economical and can be successfully applied for the estimation of thiocolchicoside in pharmaceutical dosage forms.