Comparative in vitro antioxidant activities of ethanolic extract, ethyl acetate extract (EAE), and hexane extracts (HE) of Tecoma gaudichaudi flowers

Abstract

Background: Free radicals excessive production causes biological molecules direct damage to DNA, proteins, lipids, and carbohydrates and may cause the development of tumor and its progression. Phytochemically derived natural antioxidant molecules may directly inhibit the radical production or their propagation is limited or to protect the system they will be destroyed. Objective: In the present study, in vitro antioxidant activity of ethanolic extract (EE), ethyl acetate extract (EAE), and hexane extracts (HE) of Tecoma gaudichaudi flowers was investigated. Materials and Methods: In vitro antioxidant activity of EE, EAE, and HE of T. gaudichaudi flowers was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, reducing power method, phosphomolybdenum assay and hydroxyl radical scavenging assay and IC50 values were calculated. Ascorbic acid is used as standard drug. Results: EE, EAE, and HE of T. gaudichaudi exhibited a significant antioxidant status which is evident from their IC50 values 21.42, 44.77, and 71.44 ug/mL in DPPH method and 22.3, 57.27, and 95.49 ug/mL in hydroxyl radical. In reducing power method and phosphomolybdenum assay, there is concentration-dependent increase in absorbance. The results are significant when compared to the standard drug ascorbic acid. Conclusion: EE, EAE, and HE of T. gaudichaudi flowers and specifically the EE reveal several properties such as higher free radical scavenging properties, significant antioxidant capacities compared to other extracts.