Antioxidant and antidiabetic activity of isolated flavonoids from Alangium salvifolium leaves extracts

Abstract

Background: Alangium salvifolium is content of flavonoids and mainly used for management of diabetes in India. In the present study, we evaluated the antioxidant and antidiabetic activity of isolated flavonoids from A. salvifolium leaves extracts in experimental animals. Materials and Methods: The petroleum ether, ethanol and aqueous extracts of leaves of A. salvifolium were prepared by successive Soxhlet extraction process, and further phytochemical screening and in vitro antioxidant were performed. The most effective ethanol extracts of A. salvifolium were selected for separation of flavonoids compound, and consequently qualitative phytochemical analysis was done. The flavonoid containing fractions were screened for antidiabetic activity in streptozotocin (STZ) induced diabetic rats. Results: The ethanol and aqueous extracts of A. salvifolium contain the maximum number of phytoconstituents such as alkaloids, glycosides, carbohydrates, flavonoids, tannins, and polyphenol. The findings of in vitro antioxidant activity confirmed that ethanol extracts expressed higher antioxidant activity compared to aqueous extract, and hence ethanol extracts were further selected for the isolation of various fractions. The FAS6 to FAS7 indicate the presence of phenolic compounds and flavonoids. The FAS6 and FAS7 significantly reduced the blood glucose level, total cholesterol, low-density lipoprotein, triglycerides and a significant rise in high-density lipoprotein in STZ-induced-diabetic rats as compared to the diabetic control group. Conclusion: Based on the results of the antidiabetic activity of FAS6 to FAS7 isolated from leaves of A. salvifolium were due to its antioxidant property.