Effect of time on extraction of Ashwagandha in various Hydroalcoholic compositions and their anti-inflammatory activity
DOI:
https://doi.org/10.22377/ijgp.v5i1.178Abstract
Ashwagandha, is a plant of Solanaceae or nightshade family which have botanical name Withania somnifera. It is mainly cultivated in Madhya Pradesh, Rajasthan, Uttar Pradesh and many other states of India. Among all states Madhya Pradesh (Neemuch-Mandsaur region) is the major producer of Ashwagandha. Major phytoconstituents are withanolides and alkaloids. These phytoconstituents have many pharmacological activities such as anti-cancer, diuretic, immunomodulatory, anti-inflammatory, anti-stress. The yield of activeconstituents may vary with time, temperature, number of extractions, drug and solvent ratio. Extracts were prepared in different water and alcohol compositions at different time intervals. These prepared extracts were chromatographed and number of phytoconstituents was present. Some of the extracts were used for performing anti-inflammatory activity. The activity was performed by carrageneaninduced paw oedema method in rats. A few extracts were found effective reducing the oedema. Extract obtained at 15 h was found superior in anti-inflammatory activity which is proposed due to some additional phytoconstituents extracted at that point of time.
Key words: Anti-inflammatory activity, Ashwagandha, Time
Downloads
References
Owais M, Sharad KS, Shehbaz A, Saleemuddin M. Antibacterial
efficacy of Withania somnifera (Ashwagandha) an indigenous
medicinal plant against experimental murine salmonellosis,
Phytomedicine 2005;12:229-35.
Mishra LC, Singh BB, Dagenais S. Scientific basis for the
therapeutic use of Withania somnifera (Ashwagandha): A review.
Altern Med Rev 2000;5:334-46.
SinghB, Gupta DK, Chandan BK. Adaptogenic activity of a glycopeptido-
lipid fraction from the alcoholic extract of Trichopus
zeylanicus Gaertn. Phytomedicine 2001;8:283-91.
Devi PU, Sharada AC, Solomon FE. Antitumor and radiosensitizing
effects of Withania somnifera (Ashwagandha) on a transplantable
mouse tumor, Sarcoma-180. Indian J Exp Biol 1993;31:607-11.
Agarwal R, Diwanay S, Patki P, Patwardhan B. Studies on
immunomodulatory activity of Withania somnifera (Ashwagandha)
extracts in experimental immune inflammation. J Ethnopharmacol
;67:27-35.
Patwardhan B, Hooper M. Ayurveda and future drug development.
Int J Alternat Compl Med 1992;10:9-10.
Al Hindawi MK, Al Deen HS, Nabi MH, Ismail MA. Antiinflammatory
activity of some Iraqi plants using intact rats. J
Ethnopharmacol 1989;26:163-8.
Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B.
Studies on the Immunomodulatory effects of Ashwagandha. J
Ethnopharmacol 1995;50:69-76.
Twaij HA, Elisha EE, Khalid RM. Analgesic studies on some Iraqi
medicinal plants. Int J Crude Res 1989;27:109-12.
Asthana R, Raina MK. Pharmacology of Withania somnifera
Linn(Dunal): A review. Indian Drugs 1989;26:199-204.
Dhuley JN. Adaptogenic and cardioprotective action of
Ashwagandha in rats and frogs. J Ethanopharmacol 2000;70:57-63.
Budhiraja RD, Sudhir S. Review of biological activity of
withanolides. J Sci Indus Res 1987;46:488-91.
Rahman A, Abbas A, e-Shahwar D, Jamal SA, Choudhary MI.
Abbas S. New withanolides from Withania spp. J Nat Prod
;56:1000-6.
Available from: http://portal.ics.trieste.it/MAPs/MedicinalPlants_
Plant.aspx?id=664. [cited in 2010].
Vogel HG, editor. Drug Discovery and Evaluation: Pharmacological
Assays. 2nd ed. New York:Springer; 2002.
Joshi C, Gajbhiye N, Phurailatpam A, Geetha KA, Maiti S.
Comparative morphometric, physiological and chemical studies of
wild and cultivated plant types of Withania somnifera (Solanaceae).
Curr Sci 2010;5:644-50.
Available from: http://www.holistic-herbalist.com/ashwagandha-c.
html. [accessed on 2007 Aug 24].
