Antimicrobial and wound healing activities of leaves of Alternanthera sessilis Linn
DOI:
https://doi.org/10.22377/ijgp.v2i3.18Abstract
Th e leaves of Alternanthera sessilis (Linn.) R.Br.ex DC (Family Amaranthaceae) were exhaustively extracted by soxhlet apparatus withdiff erent solvents like, petroleum ether (40-60ºC), chloroform, acetone, methanol and distilled water in ascending order of the polarity. All the fi ve extracts were subjected to antimicrobial screening by using the cup plate and turbidimetric methods. Th e chloroform extract among all fi ve extracts showed maximum zone of inhibition and signifi cant MIC values in above two methods respectively. Hence chloroform extract was taken for the screening of wound healing activity and models adopted for wound healing activity were, incision, excision and granuloma studies. Th e chloroform extract of leaves of Alternanthera sessilis at a dose of 200 μg/mL (orally) in all models showed signifi cant results. Contraction of wound area (P < 0.005) at 16th day, time for complete epithelization in 16 days (P < 0.0001), and scar area after complete epithelization was found to be 33.2 ± 0.0730 (P < 0.0008) in excision wound model, wound breaking strength 388 ± 5.85 grams (P < 0.0001) in incision wound model, granuloma dry weight 47.7 ± 2.29, granuloma breaking strength 247 ± 10.2 (P < 0.022) in granuloma studies. All the results were signifi cant for diff erent parameters in wound healing activity when compared with control group. Presence of sterols in chloroform extract was also confi rmed by preliminary phytochemical investigation, TLC and HPTLC methods.Key words: Alternanthera sessilis, antimicrobial, chloroform extract, wound healing
Downloads
References
The Wealth of India. Raw materials. Vol 1(Revised), New Delhi:
CSIR; 1985. p. 318-9.
Rastogi RP. Compendium of Indian medicinal plant. 2nd ed.
Lucknow: CDRI; 1993. p. 1970-9.
Gupta AK, Indian medicinal plants. New Delhi: ICMR; 2004. p.
-7.
Sinha P, Arora VK, Wahi SP. Chemical investigation on Alternanthera
sessilis. Indian Drug 1984;1:139-40.
Anandkumar BH, Sachidanand YN. Treatment of acne vulgaris
with new polyherbal formulation clarina cream. Indian J Dermatol
;46:1-3.
Giacomett i A, Cirioni O, Schimizzi AM, Prete DM, Barchiesi F,
Errico DM, et al. Epidemiology and microbiology of surgical
wound infections. J Clin Microbiol 2000;38:918-22.
Paul W, Sharma CP. Chitosan and alginate wound dressings: A
short review. Trends Biomater ArtiÞ cial Organs 2004;18:18-23.
Douglas M, Alan LM, Support for wound healing. Alter Med Rev
;8:359-60.
Indian Herbal Pharmacopoeia. RRR Jammu Tawai and IDMA
Mumbai: 1999. p. 47-9.
Pharmacopoeia of India, 4th ed, Vol. 1, New Delhi: Government
of India, Ministry of Health and Family Welfare; 1985. Appendix
:3:10, p. 69.
Murray PR, Baron EJ, Pfallar MA, Tenover FC, Yolke RH. Manual
of clinical microbiology. 6th ed. Washington, DC: ASM; 1995.
Organization for Economic Co-operation and Development,
revised draft guidelines 423, “OECD Guideline for the testing of
chemicals” Revised document – October 2000.
Werner S, Breededen M, Hubner G, Greenhalgh DG, Longaker MT.
Introduction of keratinocyte growth factor expression is reduced
and delayed during wound healing in the genetically diabetic
mouse. J Investig Dermatol 1994;103:469-73.
Ehrlich HP, Hunt TK. The effect of cortisone and anabolic
steroids on the tensile strength of healing wounds. Ann Surg
;167:324.
Lee KH. Studies on the mechanism of action of salicylate retardation
of wound healing by aspirin. J Pharma Sci 1968;57:1042-3.
Dispasquale G, Meli A. Eff ect of body weight changes on the
formation of cott on pellet induced granuloma. J Pharm Pharmacol
;17:379-82.
Rathi BS, Bodhankar SL, Baheti AM. Evaluation of aqueous extract
of Moringa oleifera for wound healing in albino rats. Indian J Exp
Biol 2006;44:898-901.
Nayak BS, Anderson M, Pereria Pinto LM. Evaluation of wound
healing potential of Catharanthus roseus leaf extract in rats.
Fitoterapia 2007;78:540-4.
Patil MB, Jalalpure SS, Ali A. Preliminary phytochemical
investigation and wound healing activity of leaves of Argemone
maxicana Linn. (Papaveraceae). Indian Drug 2001;38:288-93.
