In Vitro Antioxidant and Antimicrobial Activity of Polyherbal Formulation

Abstract

Context: Antioxidants play a major role in protecting the body against oxidative stress that is associated with many chronic diseases and disorders including chronic wounds. Plants are the richest source for antioxidant and are effective in the management of oxidative stress, caused by free radical damage. Wound healing and antimicrobial potential are also attributed to the antioxidant potential of drugs. Aim: The aim of this study is to carry out the antioxidant and antimicrobial activity of given polyherbal formulation (PHF) to correlate with the wound healing potential of the formulation. Materials and Methods: Antioxidant potential of the PHF was evaluated by the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. Agar well diffusion method was used to determine its antimicrobial activity against the Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella aerogenes. Results: Results of the study demonstrated that PHF exhibited significant antioxidant activity. Antibacterial activity of polyherbal formula was evaluated against the four pathogenic microorganisms in which it showed mild-to-moderate antimicrobial activity against the E. coli and K. aerogenes, while mild antimicrobial activity against the P. aeruginosa and P. vulgaris. Conclusion: Results of this study suggested that PHF can be used for the treatment of wound infections due to its marked antioxidant and antimicrobial activity.