Formulation and evaluation of herbal gel from tannin-enriched fraction of Psidium guajava Linn. leaves for diabetic wound healing

Abstract

Aim: The ethno botanical studies and folklore claiming showed that the leaves of Psidium guajava Linnare used for the treatment of wounds. From the literature review, we found that tannins have significant wound healing activity by promoting the cicatrisation of wounds and also have antimicrobial property which will speed up the wound healing process. As the wound healing process is delayed in the diabetic condition, the present work is focused on the evaluation of gel formulated at different concentrations from tannin-enriched fraction of Psidium guajava leaves for diabetic wound healing. Materials and Methods: The tannin-enriched fraction was isolated from P. guajava leaves and phytochemically evaluated by qualitative chemical test, thin-layer chromatography, and high-performance thin-layer chromatography. The total phenolic content and tannin content were estimated by colorimetric technique (Folin–Ciocalteu and Folin-Denis method). The gel was formulated and evaluated for various physiochemical properties and diabetic excision wound healing activity using male albino Wistar rats. Results and Discussion: The statistical analysis showed that there is a significant difference between diabetic control group and the group of animals treated with gel formulation (5% and 10%). The histopathological evaluation showed that the 10% gel applied a group of animals has a significant effect on diabetic wound healing activity. Conclusion: It is concluded that the gel of tannin-enriched fraction of P. guajava Linn. showed significant wound contraction at the tested concentration in diabetic condition due to the presence of gallic acid.