Standardization of quercetin in Hibiscus rosa-sinensis flower by high-performance thin-layer chromatography

Abstract

Aim: This is done from regulatory perspective to ensure the efficacy, quality, as well safety of the herbal drugs present in a plant. Materials and Methods: Standardization of plants material by high-performance thin-layer chromatography (HPTLC): HPTLC is a method to standardize and identify the chemical component which is expected to be present in a medicinal plant. This is done from regulatory perspective to ensure the quality, efficacy, and safety of the herbal drugs present in a plant. Results and Discussion: The flower of Hibiscus rosa-sinensis was dried and pulverized. The phytoconstituents of the pulverized plant material were extracted with methanol. Quercetin, reported to have anti-inflammatory, antihypertensive, vasodilator effects, antiobesity, antihypercholesterolemic, and antiatherosclerotic, etc., activities, was selected as the active biomarker for quantification of the aforementioned plant material. HPTLC was carried out for quantification. The percentage content of quercetin in H. rosa-sinensis methanolic extract was found to be 0.30% w/w by HPTLC. Conclusion: The percentage content of quercetin in methanolic extract of H. rosa-sinensis flower was found to be 0.30% w/w. This was determined by a calibration curve with the equation of Y = 3303.952*X + 2991.844.