Antioxidant and anti-inflammatory properties of G-immune plus: A polyherbal formulation

Abstract

Background: G-Immune Plus is a polyherbal formulated capsule made from the extracts of Wedelia calendulacea, Tinospora cordifolia, Withania somnifera, Centella asiatica, Emblica officinalis, Asparagus racemosus, and Tribulus terrestris. Materials and Methods: The antioxidant properties of the polyherbal formulation G-immune plus capsule were evaluated by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay, 2, 2’-azinobis-3-ethyl-benzothiozoline-6-sulfonic acid (ABTS) radical scavenging assay, total antioxidant capacity assay, ferric reducing antioxidant power assay, and copper reducing power capacity assay. Likewise, the anti-inflammatory properties were evaluated by inhibition of albumin denaturation assay and membrane stabilization assay. Results and Discussion: The antioxidant capacity and reducing activities by the antioxidants present in the formulation were observed proving the presence of antioxidant activity. The radical scavenging activity was observed with the IC50 value being 7.49 mg/ml from ABTS assay. The anti-inflammatory activity by stabilizing the membrane was found with the IC50 value being 7.02 mg/ml from membrane stabilization assay. Conclusion: The present study suggested that this polyherbal formulated capsule possess moderate to potential antioxidant and anti-inflammatory activities. Due to the presence of multiple herbs with enormous therapeutic activities within this formulation, the capsule could also exert multiple pharmacological effects on its targets and making it a potent Ayurceutical supplement for human health.