In vitro therapeutic correlative studies of Pandanus odoratissimus flowers against diabetic foot ulcer-causing bacterial pathogens

Abstract

Background: Diabetic foot ulcer (DFU) is a major secondary complication that arouses subsequently due to careless of patient in poor therapeutic management of chronic diabetes. This may sometimes lead to amputation of foot which creates mental stress and societal empathy among diabetic patients. Hence, the aim of the present investigation is formulated to evaluate and correlate the in vitro antioxidant efficacy, in vitro invertase inhibitory activity and in vitro antibacterial activity of Pandanus odoratissimus flower extracts. Materials and Methods: In vitro cupric ion reducing antioxidant capacity and 1, 1-diphenyl-2- picrylhydrazyl (DPPH) assays were performed to analyze the reducing power and radical scavenging property of P. odoratissimus flower extracts. Standard broth dilution and agar well diffusion assays were espoused for antibacterial activity. Similarly, DNS method is adopted to investigate the invertase inhibitory activity. Results and Discussion: The bacterial pattern identification studies carried out with the clinical isolates (pus and blood) of DFU patients have revealed the presence of Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The methanol (12 ± 3 mm) and acetone (16 ± 4 mm) crude extracts possessed significant zone of inhibition against E. coli, and near satisfactory control was observed against S. aureus and P. aeruginosa. Significant dipicryl ion scavenging activity (DPPH) was recorded by methanolic (90.7 ± 2%) and aqueous extracts (81.4 ± 3%). Similarly, a satisfactory cupric ion reducing property was established by the methanolic (effective dosage [ED50]: 455.3 ± 8 μg/ml) and aqueous extracts (ED50: 452.14 ± 5 μg/ml). An effective in vitro invertase inhibitory activity (62.5 ± 3%) was recorded by methanolic extract. Conclusion: The studies proved a strong correlation exist between antioxidant power, invertase inhibition, and control against DFU causing bacterial pathogens recorded by P. odoratissimus flower extracts which promise it is application as a sustainable traditional medicine against DFU.