Optimization of extraction methods for natural pigment from Lawsonia inermis

Abstract

Background: Natural extract obtained from the Henna leaves has antimicrobial and antifungal and also acts as a fluorescent compound in the gel electrophoresis. It can be coated in silk, cotton, woolen fabric, and combination. Introduction: Dye from the Henna leaves can be obtained by various methods such as alkaline extraction, ethanolic extraction, hot water extraction, Soxhlet extraction, and enzymatic extraction. It paves the alternative way for the use of synthetic dyes and also reduces the environmental hazards and health hazards caused by synthetic dyes. Materials and Methods: The three methods (hot water, ethanolic, and enzymatic extraction) were used to extract with the optimized parameters. Hot water extraction of the dye carried with the distilled water; ethanolic extraction is carried out by 1:1 ratio of water and ethanol and the enzymatic extraction is done with the help of cellulase enzyme produced by Brevibacillus borstelensis obtained from the guts of termite, collected from the residential area. Based on the concentration the pigment enzymatic extraction efficiency is approximately equal to ethanolic. Results and Discussion: While considering the cost and eco-friendly nature, enzymatic extraction is more efficient for the extraction of dye. Extracted pigment is analyzed by the results from UV-Vis spectrophotometer and Fourier-transform infrared results.