Comparison of antimicrobial activity of herbs & spices and their phytochemical determination
DOI:
https://doi.org/10.22377/ijgp.v5i3.207Abstract
The antimicrobial activities of crude methanolic extracts of five spices and herbs viz. Cuminum Cyminum (Family: Apiaceae), Nigella sativa (Family: Ranunculaceae), Pimpinella anisum (Family: Apiaceae), Trachyspermum copticum (Family: Umbelliferae), and rhizome of Zingiber officinale (Family: Zingiberaceae) were investigated by agar well diffusion method against gram-positive bacteria (Bacillus amyloliquefaciens and Staphylococcus aureus) and gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa). The antimicrobial activities of the extracts were assessed by the diameter of zone of inhibition against test strains. The extract of N. sativashowed the best inhibition against S. aureus and P. aeruginosa. The extract of Z. officinale and P. anisum showed same minimum inhibitory concentration (MIC) against all the test strains. The extract showed significant inhibitory activity against B. amyloliquefaciens, P. aeruginosa, and S. aureus. The extract of C. cyminum significantly inhibited S. aureus, B. amyloliquefaciens, and P. aeruginosa. The extract of T. copticum was effective against E. coli, B. amyloliquefaciens, and P. aeruginosa, while the highest MIC obtained against
S. aureus. The diameters of zone of inhibition obtained for standard antibiotics viz. ampicillin, erythromycin, and tetracycline at MIC values of the extracts were compared with the crude methanolic extracts and found equally or more effective against the test strains. Phytochemical analysis showed carbohydrates, inulin, alkaloids, and glycosides in all the five extracts. Flavonoids, terpenoids, tannins, reducing sugars, soluble phenols, and saponin glycosides were also detected. Steroids were absent. The organoleptic properties of the
extracts were determined. The study indicated the great potential of these extracts as effective antimicrobial agents.
Key words: Antibiotics, antimicrobial, crude methanolic extracts, inhibition, phytochemical analysis
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References
Zaika LL. Spices and herbs: Their antimicrobial activity and its
determination. J Food Safety 1988;9:97-118.
Yao J, Moellering R. Antibacterial agents. In: Murray P, Baron E,
Pfaller M, Tenover F, Yolken R, editors. Manual of clinical
microbiology. Washington, DC: ASM; 1995. p. 1281-90.
Hora SL, Nair KK. Pollution of streams and conservation of
fisheries. Proceeding of the National Institute of Sciences of India
;10:147-66.
Avato P, Tursil E, Vitali C, Miccolis V, Candido V. Allylsulfide
constituents of garlic volatile oil as antimicrobial agents.
Phytomedicine 2000;7:239-43.
Ahmad I, Aqil F. In vitro efficacy of bioactive extracts of 15
medicinal plants against ESbetaL-producing multidrug-resistant
enteric bacteria. Microbiol Res 2007;162:264-75.
Barbour EK, Al Sharif M, Sagherian VK, Habre AN, Talhouk RS,
Talhouk SN. Screening of selected indigenous plants of Lebanon
for antimicrobial activity. J Ethnopharmacol 2004;93:1-7.
Iwu MW, Duncan AR, Okunji CO. New antimicrobials of plant
origin. In: J Janick, editor. Perspectives on new Crops and new
Uses. Alexandria, VA: ASHS Press; 1999. p. 457-62.
Tomoko N, Takashi A, Hiromu T, Yuka I, Hiroko M, Munekazu I,
et al. Antibacterial activity of extracts prepared from tropical and
subtropical plants on methicillin-resistant Staphylococcus aureus. J
Health Sci 2002;48:273-6.
Sofowora A. Medicinal Plants and Traditional Medicine in Afric.
New York: John Wiley and Son Ltd; 1993. p. 150-3.
Trease GE, Evans WC. Pharmacognosy. 11th ed. London: Brailliar
Tiridel and Macmillian Publishers; 1989.
Herborne JB. Phytochemical Methods. 3rd ed. London: Chapman
and Hall Ltd. 1973. p. 135-203.
Sabulal B, Dan M, J AJ, Kurup R, Pradeep NS, Valsamma RK,
et al. Caryophyllene-rich rhizome oil of Zingiber nimmonii from
south India: Chemical characterization and antimicrobial activity.
Phytochemistry 2006;67:2469-73.
Joe M, Jayachitra J, Vijayapriya M. Antimicrobial activity of some
common spices against certain human pathogens. J Med Plants
Res 2009;3:1134-6.
Sofia PK, Prasad R, Vijay VK, Srivastava AK. Evaluation of
antibacterial activity of Indian spices against common foodborne
pathogens. Int J Food Sci Technol 2007;42:910-5.
Mekawey AA, Mokhtar MM, Farrag RM. Antitumor and
Antibacterial Activities of [1-(2-Ethyl, 6-Heptyl) Phenol] from
Cuminum Cyminum Seeds. J Appl Sci Res 2009;5:1881-8.
Singh G, Kapoor IP, Pandey SK, Singh UK, Singh RK. Studies on
essential oils: Part 10; antibacterial activity of volatile oils of some
spices. Phytother Res 2002;16:680-2.
Upadhyay RK, Dwivedi P, Ahmad S. Screening of antibacterial
activity of six plant essential oils against pathogenic bacterial
strains. Asian J Med Sci 2010;2:152-8.
Otunola GA, Oloyede OB, Oladiji AT, J Afolayan A. Comparative
analysis of the chemical composition of three spices – Allium
sativum L. Zingiber officinale Rosc. and Capsicum frutescens L.
commonly consumed in Nigeria. Afr J Biotechnol 2010;9:
-31.
Tiwari BK, Valdramidis VP, O’Donnell CP, Muthukumarappan K, Bourke P, Cullen PJ. Application of natural antimicrobials for food preservation. J Agric Food Chem 2009;57:5987-6000.
De Martino L, De Feo V, Fratianni F, Nazzaro F. Chemistry,
antioxidant, antibacterial and antifungal activities of volatile oils
and their components. Nat Prod Commun 2009;4:1741-50.
