An investigation of the antimalarial activity of Artemisia vulgaris leaf extract in a rodent malaria model
DOI:
https://doi.org/10.22377/ijgp.v5i4.213Abstract
Antimalarial activity of an organic extract of Artemisia vulgaris was evaluated in this study in terms of both antiparasitic and antidisease activities. Antiparasitic activity of the extract at three doses (250, 500, 1000 mg/kg) was assessed in vivo using the Plasmodium yoelii rodent malaria model, using distilled water as the negative control and Coartem as the positive control. Oral administration of the extract in the 4-day suppressive assay at 500 mg/kg and 1000 mg/kg significantly (P<0.01) inhibited parasitaemia by 65.16% and 51.46%, respectively. Significant (P<0.05) antinociceptive activity was observed for the extract in the hot plate test, indicating a central, supra-spinally mediated response in relieving pain. Anti-disease activity was further corroborated by increased survival of infected mice treated with the 500 mg/kg dose. The A. vulgaris extract was tolerated well by mice over a period of 14 days (assay of sub-chronic toxicity), showing no overt signs of toxicity or stress. Hepatotoxicity (evaluated in terms of serum glutamic-oxaloacetictransaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT) levels), renotoxicity (in terms of serum urea and creatinine) and haematotoxicity (in terms of total RBC, WBC and differential leukocyte counts) were also ruled out. In conclusion, A. vulgaris leaf extract is orally active, non-toxic and as a weed it has the potential to be a cheap source of plant-based antimalarial in the future.
Key words: Antimalarial activity, Artemisia vulgaris, Plasmodium yoelii, Sri Lanka, toxicity
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References
World Health Organization. World Malaria Report 2010. Geneva:
WHO Press; 2010. Available from: http://www.who.int/malaria/
world_malaria_report_2010/worldmalariareport 2010.pdf.
Bloland PB. Drug resistance in Malaria. Geneva: WHO Press; 2001.
Available from: http://www.who.int/csr/resources/publications/
drugresist/malaria.pdf.
World Health Organization. Global report on antimalarial
drug efficacy and drug resistance: 2000-2010. Geneva: WHO
Press; 2010. Available from: http://whqlibdoc.who.int/
publications/2010/9789241500470_eng.pdf.
The Malaria Elimination Group. Shrinking the Malaria Map:
A Prospectus on Malaria Elimination. The Global Health Group;
Available from: http://www.map.ox.ac.uk/PDF/MEG_
prospectus.pdf.
Jayaweera DMA. Medical Plants. Colombo, Sri Lanka: National
Science Council of Sri Lanka; 1981.
Govindaraj S, Kumari BD, Cioni PL, Flamini G. Mass propagation
and essential oil analysis of Artemisia vulgaris. J Biosci Bioeng
;105:176-83.
Rustaiyan A, Nahrevanian H, Kazemi M. A new antimalarial agent;
effect of extracts of Artemisia diffusa against Plasmodium berghei.
Pharmacogn Mag 2008;4:17.
Peters W. Chemotherapy and drug resistance in malaria. London:
Academic Press; 1970.
Langerman L, Zakowski MI, Piskown B, Grant GJ. Hot plate
versus tail flick: Evaluation of acute tolerance to continuous
morphine infusion in the rat model. J Pharmacol Toxicol Methods
;34:23-7.
Carlton JM, Angiuoli SV, Suh BB, Kooij TW, Pertea M, Silva JC,
et al. Genome sequence and comparative analysis of the
model rodent malaria parasite Plasmodium yoelii. Nature
;419:512-9.
Scuka M, Mazrzymas JW. Post synaptic potentiation and
desensitization at the vertebrate end-plate receptors. Prog
Neurobiol 1991;38:19-33.
Stewart J, Badiani A. Tolerence and sensitization to the behavioural
effects of drugs. Behav Pharmacol 1993;4:289-312.
Ratnasooriya WD, Lokupitiya EY, Dharmasiri MG. Analgesia and
sedation induced by Mucuna prurita seeds in rats. J Med Sci Res
;27:403-6.
Bertani S, Bourdy G, Landau I, Robinson JC, Esterre P, Deharo E.
Evaluation of French Guiana traditional antimalarial remedies.
J Ethnopharmocol 2005;98:45-54.
Jayasinghe CD, Udagama-Randeniya PV, Ratnasooriya WD.
in vivo antimalarial activity of aqueous root extract of Barringtonia
acutangula in mice. Pharmacogn Mag 2008;4:15.
Khan AU, Gilani AH. Antispasmodic and bronchodilator activities
of Artemisia vulgaris are mediated through dual blockade of
muscarinic receptors and calcium influx. J Ethnopharmacol
;126:480-6.
Tigno XT, de Guzman F, Flora AM. Phytochemical analysis and
hemodynamic actions of Artemisia vulgaris L. Clin Hemorheol
Microcirc 2000;23:167-75.
Tan RX, Zheng WF, Tang HQ. Biologically active substances from
the genus Artemisia. Planta Med 1998;64:295-302.
Oketch-Rabah HA, Mwangi JW, Lisgarten J, Mberu EK. A new
antiplasmodial coumarin from Toddalia asiatica roots. Fitoterapia
;71:636-40.
Novartis. International Package Leaflet. Novartis Pharma AG;
Available from: http://www.coartem.com/downloads/IPLCoartem.
pdf.
Calabrese EJ. Principles of Animal Extrapolation. USA: Lewis
Publishers Inc; 1991.
