Pharmacognostic evaluation of an ayurvedic formulation Eladi Gutika
DOI:
https://doi.org/10.22377/ijgp.v5i4.218Abstract
Eladi Gutika (EG), a traditional Ayurvedic polyherbal formulation is used as a remedy for Kasa (Cough), Svasa (Asthma),Bhrama (Vertigo), Raktapitta (Bleeding disorders), Jvara (Fever), Amvata (Rheumatism) etc. In the present work, an attempt
has been made to develop pharmacognostic standards for EG. The raw materials of EG were subjected to proximate analysis
prior to preparation of EG. EG was prepared using raw materials of pharmacopoeial quality. Powder microscopy of EG showed
the presence of discerning anatomical characters which were present in the raw materials. Preliminary phytochemical evaluation of EG revealed the presence of carbohydrates, alkaloids, flavonoids, essential oils, glycosides and tannins. A simple, rapid, accurate and sensitive HPTLC method was developed and validated for the quantitation of piperine from EG. Method was validated as per ICH guidelines and applied for stability studies of EG stored for different storage periods at room temperature. A comparative evaluation of EG prepared in-house was carried out with three available marketed samples in terms of their respective piperine content. Acute toxicity study of EG in Albino Swiss mice revealed that it is safe at the dose of 2 g/kg body weight in animal. Evaluation of EG by these scientific methods can be used as quality control tool for the manufacturing and processing of EG.
Key words: Eladi gutika, HPTLC, piperine, quality control, standardization
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References
Shailajan S, Yeragi M, Purohit A. Optimized separation and
quantification of eugenol from a traditional Unani medicine
Jawarish-e-Bisbasa using HPTLC. Int J Pharm Sci Rev Res 2011;9:
-51.
Shailajan S, Singh A, Tiwari B. Quality control and standardization
of an ayurvedic Taila formulation. Int J Biomed Res Anal 2010;1:
-81.
Shialajan S, Menon S. Polymarker based standardization of an
ayurvedic formulation Lavangadi Vati using high performance thin
layer chromatography. J Pharm Res 2011;4:467-70.
Shailajan S, Menon S, Singh A. Quantitative analysis of piperine
from ayurvedic polyherbal formulations using reverse phase
high performance liquid chromatography. Int J Pharma Biosci
;1:1-10.
Ayurvedic Formulary of India (AFI), Part I. Government of India,
Ministry of Health and Family Welfare. New Delhi: Department
of Health, Controller of Publications Civil Lines; 1989. p. 181-2.
Mukherjee PK. Quality control of herbal drugs - An approach to
evaluation of botanicals. New Delhi: Business Horizons Publishers;
Pharmacopoeia of India, (IP). 3rd ed. New Delhi: Government of
India; 1985.
Khandelwal KR. Practical pharmacognosy techniques and
experiments. 19th ed. Pune: Nirali Prakashan Publishers; 2008.
Tapadiya G, Metku M, Deokate U, Khadabadi S, Saboo S, Sahu K.
Quantitative estimation of piperine from pharmaceutical dosage
form by HPTLC. Asian J Pharm Cli Res 2009;2:47-50.
Bajada S, Singla AK, Bedia KL. Liquid chromatographic method
for determination of piperine in rat plasma: Application to
pharmacokinetics. J Chromatogr B 2002;776:245-9.
Capasso R, Izzo AA, Borrelli F, Russo A, Sautebin L, Pinto A, et al.
Effect of piperine, the active ingredient of black pepper on intestinal
secretion in mice. Life Sci 2002;71:2311-7.
Hamrapurkar PD, Jadhav K, Zine S. Quantitative estimation of
piperine in Piper nigrum and Piper longum using high performance
thin layer chromatography. J Appl Pharm Sci 2011;1:117-20.
Patel RK, Kanani RJ, Patel VR, Patel MG. Development and
validation of HPTLC method for simultaneous quantification
of vasicine and piperine in Vasavaleha. Int J Pharm Res 2010;2:
-7.
ICH. Validation of analytical procedures: Methodology. ICH
harmonized tripartite guideline. Available from:
hc-sc.gc.ca/hpb-dgps/therapeut> [Last accessed on 2004 Apr 07].
Evans WC. Trease and evans pharmacognosy. 14th ed. London: SW
Saunders Company Ltd.; 1996. p. 319.
OECD. Revised analysis of responses received from member
countries to the questionnaire on data requirements for acute oral
toxicity. Environmental health and safety monograph series on
testing and assessment No. 26; 1999.
Parameshwaran S, Nandan DM. Standardisation of an ayurvedic
formulation “Sanjivani Vatiâ€. Int J Green Pharm 2010;4:153-5.
Rashmibala S, Kumar SP. Standardization of Lasunadi Vati:
An ayurvedic polyherbal formulation. Int J Pharm World Res
;2:1-9.
