Isolation and diagnosis of phytochemicals extracted from pomegranate husks and study of the biological effect of extract (O-Veratramide) as an antimicrobial activity

Abstract

Context: The biological efficiency of O-veratramide (2,3-dimethoxybenzamide) isolated from pomegranate extract was studied. Extraction of 400 g of dried pomegranate husks was carried out by Soxhlet, and seven organic compounds were extracted using a mixture of organic solvents. O-veratramide has been deposited and isolated. Several solvents such as methanol, chloroform, ethyl acetate, and hexane have been used. The extraction ratio was higher when using methanol and less in chloroform. Objective: The biological efficacy of O-veratramide was investigated against different types of bacteria by taking the highest concentration of this compound and less concentration of the compound. Materials and Methods: The purpose of the manuscript was to study the inhibitory activity of the bacteria using MacConkey Agar as an agro-medium. O-veratramide showed excellent bioavailability against different types of Gram-negative and positive bacteria. Results: All extracts were analyzed by the GC-Mas technique to identify and distinguish the chemical compounds present in the raw extract in both organic and biological layers. A number of internationally recognized detection methods have been used to detect each type of compound extracted. The spectral analysis of the O-veratramide was studied, and its biological efficacy was studied against Gram-negative and positive bacteria. Conclusions: GC-Mas technique revealed the presence of seven compounds in the pomegranate extract. The same technique has demonstrated the possibility of isolating the O-veratramide using different solvents, its potential for estimation, and the study of its biological effect.