Evaluating the nephron protective activity of Argyreia nervosa wholeplant against gentamicin induced nephrotoxicity in albino Wister rats

Abstract

Objective: The objective of the present study is to develop the pharmacological activity of the herbal drug using ethanolic extract of whole plant of Argyreia nervosa which is claimed for its effective treatment of ailments with low toxicity and economic viability. Collection and authentication of whole plant A. nervosa. Extraction of leaves of A. nervosa with 90% ethanol. Phytochemical screening of ethanolic extract of A. nervosa whole plant. Pharmacological evaluation of ethanolic extract biochemical parameters such as estimation of levels of creatinine in blood serum, estimation of levels of urea in blood serum, estimation of levels of uric acid in blood serum and Statistical analysis. Materials and Methods: Picric acid, alkalin buffer reagent, standard creatinine, surfactant, urea reagent, acid reagent, diaceylmonoxime reagent, urea standard, buffer reagent, enzyme reagent, uric acid standard, A. nervosa, gentamicin, di-ethyl ether. Tests for flavonoids, tests for steroids, tests for alkaloids, tests for tannins, tests for carbohydrates, tests for cardiac glycosides, tests for anthra quinones glycosides, tests for amino acids, test for phenols, acute oral toxicity studies, experimental design: The animal experiments were performed after acclimatization animals were separated in to four groups of each group with 6 animals were placed in separate cages, collection of blood, estimation of serum creatinine, estimation of uric acid, estimation of urea, statistical report. Conclusion: The presence of flavonoids, tannins, steroids, carbohydrates, phenols etc. In acute toxicity studies the test dose 200 mg/kg and 400 mg/kg was chosen as experimental doses. Nephroprotective activity of ethanolic extract of A. nervosa was screened for biochemical parameters (serum creatinine, serum uric acid, serum urea). The ethanolic extracts have produced effective nephroprotective action as evident by results obtained from the studies. The interpretation of the results was done after subjecting the data obtained from various studies by simple graph. The studies indicate that ethanolic extract of A. nervosa whole plant producing promising results in treatment of nephrotoxicity