Determination of bacoside A by HPTLC in Bacopa monnieri extract
DOI:
https://doi.org/10.22377/ijgp.v2i3.26Abstract
A simple sensitive HPTLC method developed for the determination of bacoside A in the plant Bacopa monnieri extracts. Th e stationaryphase was precoated silica gel GF254. Th e mobile phase used was dichloromethane: methanol: water (4.5: 1.0: 0.1 v/v/v). Th e plate
was scanned and quantifi ed at 225 nm for bacoside A. Th e method was validated in terms of linearity, accuracy and specifi city. Th e proposed HPTLC method provides a faster and cost eff ective qualitative control for routine analysis of bacoside A in extracts containing Bacopa monnieri saponins.
Key words: Analysis, bacoside A, HPTLC
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References
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