Extraction and pharmacological evaluation of flavonoids of sida acuta Burm. f.

Authors

  • Alka Jindal
  • Padma Kumar
  • Geeta Singh

DOI:

https://doi.org/10.22377/ijgp.v6i3.263

Abstract

Background: Flavonoids are group of polyphenolic compounds which are known to have medicinal properties and play a major
role in the successful medicinal treatments from ancient times. Aims: The present study is conducted to evaluate the antimicrobial
activity of free and bound flavonoids of Sida acuta Burm. f. Materials and Methods: The disc diffusion assay was performed against
three bacteria (Escherichia coli, Staphylococcus aureus and Proteus mirabilis) and two fungi (Aspergillus flavus and Aspergillus niger).
Minimum inhibitory concentrations were evaluated by the micro broth dilution method, while minimum bactericidal/fungicidal
concentrations were carried out by subculturing the relevant samples. Total activity was also calculated for the extracts, to relate minimum inhibitory concentration of the extract with its amount isolated from 1 g of dried plant material. Statistical Analysis: All the values of the results of disc diffusion assay were expressed as means of three replicates ± standard error means. Results: The flavonoid extracts showed only antibacterial activity, but were inactive against the tested fungi. Free flavonoids from roots and bound flavonoid from stems exhibited antibacterial activity against all tested bacteria. S. aureus was the most susceptible microorganism which was sensitive toward 6 extracts out of 7 active extracts followed by P. mirabilis (5 extracts) and E. coli (3 extracts), respectively.
Conclusion: The antibacterial flavonoids from S. acuta could be used in developing novel antibacterial drugs.
Key words: Antimicrobial activity, flavonoid, sida acuta, total activity

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References

Inamdar N, Edalat S, Kotwal VB, Pawar S. Herbal drugs in milieu

of modern drugs. International J Green Pharm 2008;2:2‑8.

Silver LL, Bostian KA. Discovery and development of new

antibiotics: The problem of antibiotic resistance. Antimicrob

Agents Chemother 1993;37:377‑83.

Renzo AC, Silvana SF, Cristiane YI, Antonio OC. Antimicrobial

activity of Uncaria tomentosa against oral human pathogens.

Brazilian Oral Res 2007;21:46‑50.

Al‑Bakri GA, Afifi UF. Evaluation of antimicrobial activity of

selected plant extracts by rapid XTT colorimetry and bacterial

enumeration. J Microbiol Method 2007;68:19‑25.

Abere TA, Onyekweli AO, Ukoh GC. In vitro antimicrobial activity

of the extract of Mitracarpus scaber leaves formulated as syrup. Trop

J Pharm Res 2007;6:679‑82.

Barrett B. Medicinal plants of Nicaragua’s Atlantic Coast. Econ Bot

;48:18‑20.

Coee FG, Anderson G. Screening of medicinal plants used by

the Garifuna of Eastern Nicaragua for Bioactive Compounds.

J Ethnopharmacol 1996;53:29‑50.

Kholkute SD, Munshi SR, Naik SD, Jathar VS. Antifertility activity

of indigenous plants Sida carpinifolia Linn and Podocarpus

brevifolius Stapf on female rats. Indian J Exp Biol 1978;16:696.

Malairajan P, Gopalakrishnan G, Narasimhan S, Veni KJ. Analgesic

activity of some Indian medicinal plants. J Ethnopharmacol

;106:425‑8.

Wake RR. In vitro antimicrobial activity of extracts of plants of

genus Sida Linn. Int J Pharma Res Dev 2011;3:210‑4.

Ibrahim TA, Adetuyi FO, Ajala L. Phytochemical screening and

antibacterial activity of Sida acuta and Euphorbia hirta. J Appl

Phytotechnol Environ Stud 2012;1:113‑9.

Sofowara A. Medicinal plants and Traditional medicine in Africa.

Ibadan, Nigeria: Spectrum Books Ltd.; 1993. p. 289.

Harborne JB. Phytochemical methods. London: Chapman and

Hall Ltd; 1973. p. 49‑188.

Subramanian SS, Nagarjan S. Flavonoids of the seeds of Crotolaria

retusa and Crotolaria striata. Curr Sci 1969;38:65.

Andrews JM. BSAC standardized disc susceptibility testing

method. J Antimicrob Chemother 2001;4:43‑57.

Basri DF, Fan SH. The potential of aqueous and acetone extracts

of galls of Quercus infectoria as antibacterial agents. Indian J

Pharmacol 2005;37:26‑9.

Eloff JN. Quantifying the bioactivity of the plant extracts during

screening and bioassay‑guided fractionation. Phytomedicine

;11:370‑1.

Jang DS, Park EJ, Kang YH, Su BN, Hawthorne ME, Vigo JS,

et al. Compounds obtained from Sida acuta with the potential to

induce quinone reductase and to inhibit 7, 12‑ dimethylbenz[a]

anthracene‑induced preneoplastic lesions in a mouse mammary

organ culture model. Arch Pharmacol Res 2003;26:585‑90.

Edeoga HO, Okwu DE Mbaebie BO. Phytochemical constituents

of some Nigerian medicinal plants. Afr J Biotech 2005;

:685‑8.

Alanis SAD, Glazada F, Gerrantes JA, Tarres J, Ceballas GM.

Antibacterial properties of some plants used in mexican

traditional medicine for the treatment of gastrointestinal disorders.

J Ethnopharmcol 2005;100:153‑4.

Anani K, Hudson JB, De Souza C, Akpkagana K, Tower GH,

Amason JI, et al. Investigation of medicinal Plants Togo for antiviral

and antimicrobial activities. Pharmaceutical Biol 2000;38:40‑5.

Saganuwa AS, Gulimbe ML. Evaluation of Sida acuta subspecies

leaf/flower extract for antimicrobial activity and phytochemical

constituents. Afr J Exp Microbiol 2006;7:83‑8.

Ekpo MA, Etim PC. Antimicrobial activity of ethanolic and

aqueous extracts of Sida acuta on micro organisms from skin

infections. J Med Plant Res 2009;3:621‑4.

Oboh IE, Akerele JO, Obasuyi O. Antimicrobial activity of the

ethanol extract of the aerial parts of Sida acuta burm.f. (malvaceae).

Trop J Pharma Res 2007;6:809‑13.

Pongpan A, Chumsri P, Taworasate T. The antimicrobial activity

of some Thai Medicinal Plants. Mahidol Univ J Pharm Sci

;9:88‑91.

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