In vitro embryo culture and antimicrobial activity of Clitoria ternatea L.
DOI:
https://doi.org/10.22377/ijgp.v6i4.281Abstract
Background: Clitoria ternatea L. is an important rare medicinal plant species with memory‑enhancing ability used as crude drugsin many ayurvedic medicines. Objective: The objectives were as follows: A. To develop a protocol for rapid clonal propagation
of the important medicinal climber, C. ternatea L., through in vitro tissue culture of embryo explants through callogenesis and
organogenesis, and B. Antibacterial study of ethanolic extract of in vitro raised plant and callus mass against Pseudomonas
aeruginosa (MTCC189), Bacillus subtilis (MTCC8), Escherichia coli (MTCC1), and Klebsiella pneumonia (MTCC3883), respectively.
Materials and Methods: Explants were cultured on Murashige and Skoog’s (MS) medium supplemented with different concentrations and combinations of 6‑benzylamino purine (BAP), (2,4‑dichlorophenoxy acetic acid (2,4‑D), and a‑naphthalene acetic acid (NAA)for shoot and root induction. The disc diffusion method was adopted for antimicrobial study of the plant extract. Results: The sub‑cultured of callus on MS basal medium supplemented with BAP (2.5 mg/l) and NAA (0.5 mg/l) showed highest rate of shoot multiplication. In vitro shoots were rooted on to the MS basal medium supplemented with NAA (0.5 mg/l). The sub‑culture of callus on MS basal medium supplemented with BAP (1 mg/l) and NAA (0.5 mg/l) showed highest rate of root multiplication from callus. The antibacterial activity of the ethanolic extracts of in vitro grown products of C. ternatea L. was found to have antimicrobial activity
against all tested microorganisms. Preliminary phytochemical screening revealed the presence of alkaloids, flavonoids, saponins,carbohydrates, and steroids in the in vitro grown products of C. ternatea L. Conclusion: An efficient protocol was developed for successful micropropagation and multiple plant regeneration of an important medicinal plant C. ternatea L. It is a widely used in ayurvedic medicine because of its multi‑potent bioactive molecules and significant antibacterial properties.
Key words: Antibacterial activity, Clitoria ternatea L., ethanolic extracts, growth regulator, in vitro multiplication, phytochemicals
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References
Korosecchviz, JI, Howe‑Grant, Kirk‑Othmer M. Encyclopedia of
Chemical Technology, Wiley, New York, 1992;2: 893.
Madhu K. Evaluation of ethanolic extracts of in vitro grown Bauhinia
variegata L. for antibacterial activities. Int J Pharm Bio Sci 2012;4:43‑50.
Kaur GJ, Arora DS. Antibacterial and phytochemical screening of
Anethum graveolens, Foeniculum vulgare and Trachyspermum ammi.
Complement Alternat Med 2009;9:30.
Samy RP, Ignacimuthu S, Sen A. Screening of 34 Indian
medicinal plants for antibacterial properties. J Ethnopharmacol
;62:173‑81.
Hamilton AC. Medicinal plants: Conservation and livelihoods.
Biodivers Conserv 2004;13:1477‑517.
Khadatkar SN, Manwar JV, Bhajipale NS. In vitro anthelmintic
activity of root of Clitoria ternatea Linn. Pharmacogn Mag
;4:148‑50.
Devi BP, Boominathan R, Mandal SC. Anti‑inflammatory, analgesic
and antipyretic properties of Clitoria ternatea root. Fitoterapia
;74:345‑9.
Jain NN, Ohal CC, Shroff SK, Bhutada RH, Somani RS, Kasture VS,
et al. Clitoria ternatea and the CNS. Pharmacol Biochem Behav
;75:529‑36.
Sharma AK, Majumdar M. Some observations on the effect of
Clitoria ternatea Linn. on changes in serum sugar level and small
intestinal mucosal carbohydrase activities in alloxan diabetes.
Calcut Med J 1990;87:168‑71.
Balachandran P, Govindarajan R. Cancer‑an ayurvedic perspective.
Pharmacol Res 2005;51:19‑30.
Harish MC, Rajeev Kumar S, Sathish Kumar R. Efficient in vitro
callus induction and regeneration of different tomato cultivars of
India. Asian J Biotech 2010;2:178‑84.
Mukherjee PK, Kumar V, Kumar NS, Heinrich M. The ayurvedic
medicine Clitoria ternatea from traditional use to scientific
assessment. J Ethnopharmacol 2008;120:291‑301.
Kamkaen N, Wilkinson JM. The antioxidant activity of Clitoria
ternatea flower petal extracts and eye gel. Phytother Res
;23:1624‑5.
Anis M, Faisal M. In vitro regeneration and mass multiplication
of Psoralea corylifolia an endangered medicinal plant. Ind J Biotech
;4:261‑4.
Chand S, Singh KA. In vitro shoot regeneration from cotyledonary
node explants of a multipurpose leguminous tree Pterocarpus
marsupium Roxb. In Vitro Cell Dev‑PL 2004;40:167‑70.
Snedeeor GW. Cochran in “Statistical Methods.†New Delhi:
Oxford and IBH Publishing Co; 1967. p. 33.
Anonyoums. Indian Medicinal Plants, Vol. 2. Madras: Orient
Longman; 1995. p. 129.
Osborn RW, De Samblanx GW, Thevissen K, Goderis I, Torrekens S,
Van Leuven A. Isolation and characterization of plant residues as
major sites of arylation in vitro. Chem Biol Interact 1984;48:349‑66.
Rai KS, Murthy KD, Karanth KS, Rao MS. Clitoria ternatea (Linn)
root extract treatment during growth spurt period enhances
learning and memory in rats. Indian J Physiol Pharmacol
;45:305‑13.
Ivanova D, Gerova D, Chervenkov T, Yankova T. Polyphenols
and antioxidant capacity of Bulgarian medicinal plants.
J Ethnopharmacol 2005;97:145‑50.
Raageeva B, Madhu K, Shahnawaz M, Niti K. In vitro plant
regeneration in Butterfly pea, Clitoria ternatea L. Columbian J Life
Sci 2011;12:23‑5.
Malabadi RB, Nataraja K. Shoot regeneration in leaf explants
of Clitoria ternatea L. cultured in vitro. Phytomorphology
;51:169‑71.
Rout GR. Micropropagation of Clitoria ternatea L. (Fabaceae)‑ An
important medicinal plant. In Vitro Cell Dev‑PL 2005;41:516‑9.
Murashige T, Skoog F. A revised medium for rapid growth
and bioassays with tobacco tissue cultures. Physiol Plant
;15:473‑97.
Beyer H, Walter W. Organic Chemistry (D. Llyod, translator and
Ed.) Albion publishing Ltd., Chichester, UK, 1997. p. 1037
Trease GE, Evans WC. Pharmacognosy. 15th ed. London: Saunders
Publishers; 2002. p. 221‑9.
Sofowora A. Medicinal Plants and Traditional Medicinal in Africa.
nd ed. Nigeria: Spectrum Books Ltd; Screening Plants for Bioactive
Agents; 1993. p. 134‑56.
Arvind KS, Gaurav SS, Acharya B. A rapid and simple scheme
for the standardization of polyherbal drugs. Int J Green Pham
;3:134‑40.
Jones AM, Im K‑H, Savka MA, Wu M‑K, De Will NG, Shillito R,
et al. Auxin‑dependent cell expansion mediated by overexpressed
auxin‑ binding protein‑1. Science 1998;282:1114‑7.
Johari BM, Nag KK. Morphogenic studies on endosperm of some
parasitic Angiosperms. Phytomorphology 1971;21:202‑18.
Doss A, Vijayasanthi M, Parivuguna V, Venkataswamy R.
Antimicrobial effects of flavonoid fractions of Mimosa pudica L.
leaves. J Pharma Res 2011;4:1438‑9.
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility
testing by a standardized single disk method. Am J Clin Pathol
;36:493‑6.
Doss A, Parivuguna V, VijayaSanthi M, Surendran S.
Antibacterial and preliminary phytochemical analysis of
Medicago sativa L. against some microbial pathogens. Indian J
Sci Technol 2011;4:550‑2.
Gopish Khanna V, Kannabiran K. Antimicrobial activity of saponin
fractions of the leaves Gymnema sylvestre and Eclipta prostrata.
World J Microbiol Biotechnol 2008;24:2737‑40.
