Comparison between high performance liquid chromatography and high performance thin layer chromatography determination of Diosgenin from fenugreek seeds
DOI:
https://doi.org/10.22377/ijgp.v6i4.282Abstract
Background: Fenugreek is used in the treatment of many diseases as reported in Ayurveda. The chemical like Diosgenin is one of the important chemicals of fenugreek. Aim: To develop simple, rapid, accurate methods for estimation of Diosgenin fromfenugreek using high performance thin layer chromatography (HPTLC) and high performance liquid chromatography (HPLC).
Materials and Methods: The two chromatographic methods, HPTLC and HPLC, were developed and compared to quantitative
determination of Diosgenin in fenugreek seed extract. For both the analyses, the scanning wavelength was set at 205 nm. The Diosgenin content of seven different extracts collected from different locations was compared by HPLC and HPTLC. Statistical Analysis: The relative standard deviation and correlation coefficient (r2) were calculated by standard Formulae. Results: The comparison was done in HPTLC and HPLC results and all the results are found to be within the limits and showed no significant difference. Conclusion: Both the methods were accurate and specific for the analysis of Diosgenin.
Key words: Diosgenin, fenugreek, high performance liquid chromatography, high performance thin layer chromatography,
quantitation
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References
Bin‑Hafeez B, Haque R, Parvez S, Pandey S, Sayeed I,
Raisuddin S. Immunomodulatory effects of fenugreek (Trigonella
foenum graecum L.) extract in mice. Int Immunopharmacol
;3:257‑65.
Gopu CL, Gilda SS, Paradkar AR, Mahadik KR. Development and
validation of a densitometric tlc method for analysis of trigonelline
and 4‑hydroxyisoleucine in fenugreek seeds. Acta Chromatogr
;20:709‑19.
Srinivasan K. Fenugreek (Trigonella foenum‑graecum): A review
of health beneficial physiological effects. Food Rev Int
;22:203‑24.
Onweluzo JC, Obanu ZA, Onuha KC. Functional properties of
some lesser tropical legumes. J Food Sci Tech 1994;31:302‑06.
Acharya S, Srichamroen A, Basu S, Ooraikul B, Basu T. Improvement
in the nutraceutical properties of fenugreek (Trigonella
foenum‑graecum L.). J Sci Technol 2006;28:1‑9.
Chatterjee S, Prasad SV, Sharma A. Bioactive lipid constituents of
fenugreek. Food Chem 2010;119:349‑53.
Shishodia S, Aggarwal BB. Diosgenin inhibits osteoclastogenesis,
invasion, and proliferation through the downregulation of Akt,
I kappa B kinase activation and NF‑kappa B‑regulated gene
expression. Oncogene 2006;25:1463‑73.
Liagre B, Vergne‑Salle P, Corbiere C, Charissoux JL, Beneytout JL.
Diosgenin, a plant steroid, induces apoptosis in human rheumatoid
arthritis synoviocytes with cyclooxygenase‑2 overexpression.
Arthritis Res Ther 2004;6:R373‑83.
Mehrafarin A, Qaderi A, Rezazadeh S, Naghdi BH,
How to cite this article: Nagore DH, Patil PS, Kuber VV. Comparison
between high performance liquid chromatography and high performance thin
layer chromatography determination of Diosgenin from fenugreek seeds. Int
J Green Pharm 2012;6:315-20.
Source of Support: Nil, Conflict of Interest: None declared.
Noormohammadi G. Bioengineering of important secondary
metabolites and metabolic pathways in fenugreek (Trigonella
foenum‑graecum L.). J Med Plants 2010;9:1‑18.
Anonymous. The Ayurvedic Pharmacopoeia of India. New Delhi:
Controller of publications, Government of India; 1999.
p. 112‑3.
Cardellina JH 2nd. Challenges and opportunities confronting
the botanical dietary supplement industry. J Nat Prod
;65:1073‑84.
ICH. International Conference on Harmonization Q2B: Validation
of Analytical Procedures. US FDA Fed Reg; 1997. p. 463‑7.
Chopra S, Ahmad FJ, Khar RK, Motwani SK, Mahdi S, Iqbal Z,
et al. Validated high‑performance thin‑layer chromatography
method for determination of trigonelline in herbal extract and
pharmaceutical dosage form. Anal Chim Acta 2006;577:46‑51.
