Investigation on antioxidant and antimicrobial properties of methanolic extract of Combretum indicum leaf

Abstract

Aim: Study with the medicinal plant is growing to be familiar with the value in a healthy life. A traditional medicinal plant Combretum indicum leaf was selected to investigate its antioxidant and antimicrobial potential. Materials and Methods: The leaf extract was prepared with methanol solvent. Antioxidant potentiality was assessed by free radical scavenging assay with 2,2-diphenyl-1-picrylhydrazyl (DPPH) using ascorbic acid (AA) as standard and reducing power capacity was examined by the development of Perl’s Prussian method with AA as standard. Antimicrobial activity was investigated by the method of disc diffusion with standard antibiotic Kanamycin. Results: The antioxidant activity in DPPH radical scavenging assay showed that the plant extract exhibits a dose-dependent scavenging of DPPH with IC50 48.87 μg/ml while in standard AA showed an IC50 value of 21.14 μg/ml. In reducing power capacity assay, the extract was put on view moderate action in a dose-dependent way. In antimicrobial assessment, it was observed that the extract was active against a number of the Gram-positive microorganisms and Gram-negative microorganisms exercised in the study. Kanamycin was engaged as standard, which demonstrated very high action compared to the extract. Conclusion: The observed therapeutic potentiality encourages further in-depth investigation to understand and explain possible mechanisms of action of C. indicum extract as well as to consider this plant as a potential source of alternative medicines.