Preliminary phytochemical investigation and thin layer chromatography profiling of sequential extracts of Moringa oleifera pods
DOI:
https://doi.org/10.22377/ijgp.v7i1.295Abstract
Context: Moringa oleifera Lam (Moringaceae) is a highly valued plant, distributed in many countries of the tropics and subtropics.It has an impressive range of medicinal uses with high nutritional value. Aim: The present study, primarily aims to carry out a
preliminary phytochemical screening so as to detect the major class of compounds present in M. oleifera and to perform thin layer chromatography (TLC) profiling of all sequential extracts. Materials and Methods: Phytochemical analysis was performed by various qualitative methods and TLC profiling was carried out using various solvent system of varying polarity. Results and Conclusions: Qualitative phytochemical analysis reflects the presence of phenolics, triterpenoids, cardiac glycosides, steroid, alkaloids and saponin in the plant extract. TLC profiling of the M. oleifera pods was carried out using sequential extracts of petroleum ether, benzene, petroleum ether, benzene, chloroform, ethyl acetate, ethanol and water respectively. The results obtained in the present investigation
indicated M. oleifera pods as a rich source of natural antioxidants.
Key words: Moringa oleifera, phytochemical screening, retention factor, thin layer chromatography profiling
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References
Cragg SJ, Nicholson C, Kume‑Kick J, Tao L, Rice ME.
Dopamine‑mediated volume transmission in midbrain is regulated
by distinct extracellular geometry and uptake. J Neurophysiol
;85:1761‑71.
Pavia SR, Kapln DE, Gottlieb OE. Systematic positioning of
Plumbaginaceae. Acad Bras Cienc 1995;67:461‑6.
Anwar F, Latif S, Ashraf M, Gilani AH. Moringa oleifera: A food
plant with multiple medicinal uses. Phytother Res 2007;21:
‑25.
Paliwal R, Sharma V, Pracheta. A review on horse radish
tree (Moringa oleifera): A multipurpose tree with high economic
and commercial importance. Asian J Biotechnol 2011;3:317‑28.
Paliwal R, Sharma V, Pracheta, Sharma S, Yadav S, Sharma SH.
Antinephrotoxic effect of administration of Moringa oleifera Lam
in amelioration of DMBA‑induced renal carcinogenesis in swiss
albino mice. Biol Med 2011;3:27‑35.
Paliwal R, Sharma V, Pracheta, Sharma SH. Hepatoprotective
and antioxidant potential of Moringa oleifera pods against
DMBA‑induced hepatocarcinogenesis in male mice. Int J Drug
Dev Res 2011;3:128‑38.
Sutar NG, Bonde CG, Patil VV, Narkhede SB, Patil AP, Kakade RT.
Analgesic activity of seeds of Moringa oleifera Lam. Int J Green
Pharm 2008;2:108‑10.
Sharma V, Paliwal R, Janmeda P, Sharma S. Renoprotective effects
of Moringa oleifera pods in 7,12‑dimethylbenz a anthracene‑exposed
mice. Zhong Xi Yi Jie He Xue Bao 2012;10:1171‑8.
Sharma V, Paliwal R, Janmeda P, Sharma S. Chemopreventive
efficacy of Moringa oleifera pods against 7, 12‑dimethylbenz a
anthracene induced hepatic carcinogenesis in mice. Asian Pac J
Cancer Prev 2012;13:2563‑9.
Fahey JW. Moringa oleifera: A review of the medical evidence for
its nutritional, therapeutic and prophylactic properties. Part 1.
Trees Life J 2005;1.
Bennett RN, Mellon FA, Foidl N, Pratt JH, Dupont MS, Perkins L,
et al. Profiling glucosinolates and phenolics in vegetative and
reproductive tissues of the multi‑purpose trees Moringa oleifera L.
(horseradish tree) and Moringa stenopetala L. J Agric Food Chem
;51:3546‑53.
Elango R, Jadhav U. Phytochemical screening of Moringa oleifera
using high performance thin layer chromatography. Plant Arch
:749‑51.
Harborne JB. Photochemical Methods: A Guide to Modern
Techniques of Plant Analysis. London: Chapman A. and Hall;
p. 279.
Trease GE, Evans WC. Pharmacology. 11th ed. London: Bailliere
Tindall Ltd.; 1978. p. 60‑75.
Sofowora A. Medicinal Plants and Traditional Medicines in Africa.
New York: Chichester John Wiley and Sons; 1993. p. 97‑145.
Evans WC. Treasae and Evan pharmacognosy. 14th ed. Singapor:
Harcout Brace company, Asia Pvt Ltd.; 1999. p. 343‑6.
Kokate CK. Practoca Pharmacognosy. 4th ed. New Delhi, India:
Vallaph Prakash Publication; 1999. p. 115.
Mace ME. Histochemical localization of phenols in healthy and
diseased tomato roots. Phytochem 1963;16:915‑25.
Talukdar AD, Choudhury MD, Chakraborty M, Dutta BK.
Phytochemical screening and TLC profiling of plant extracts of
Cyathea gigantea (Wall. Ex. Hook.) Haltt. and Cyathea brunoniana.
Wall. ex. Hook.(Cl. and Bak.). Assam Uni J Sci Technol Biol Env
Sci 2010;5:70‑4.
Paliwal R, Sharma V, Pracheta, Sharma, S. Elucidation of free
radical scavenging and antioxidant activity of aqueous and
hydro‑ethanolic extracts of Moringa oleifera pods. Res J Pharm Tech
;4:566‑71.
Sharma V, Paliwal R, Pracheta, Sharma S. Phytochemical
analysis and evaluation of antioxidant activities of hydroethanolic
extracts of Moringa oleifera Lam. Pods. J Pharm
Res 2011;4:554‑7.