Effect of methanolic extract of Corchorus trilocularis Linn. (Tiliaceae) in diabetic nephropathy with special reference to antioxidant activity

Abstract

Aim: Effect of methanolic extract of Corchorus trilocularis Linn. in diabetic nephropathy (DN) with special
reference to anti-oxidant activity. Materials and Methods: C. trilocularis Linn. were collected from local
medicinal garden of college campus and authentified by botanist. Dried leaves of selected plant were successively
extracted using petroleum ether, dichloromethane, ethyl acetate, methanol, and finally water. In the previous
study, methanolic extract showed potent activity so we have further selected methanolic extract for the other
model. Animals were fasted for overnight then a single intra peritoneal injection of freshly prepared streptozotocin
(50 mg/kg dissolved in citrate buffer pH 4.5) was injected and Nephropathy was induced by Ligation of the left
renal artery. Blood was collected on 0, 14, and 28 day by retro-orbital on 28 days from eye for the estimation of
biochemical parameters. The withdrawn blood was centrifuged at 5000 rpm for 10 min by using centrifuge and
plasma was collected and different biochemical parameters were determined by auto analyzer. Some antioxidant
parameters, that is, Malondialdehyde, glutathione, Catalase, and superoxide dismutase were also determined.
Results: Here body weight of rat is decreased in DN group in comparison to normal control group while increased
significantly in extract treated group at 28th day in comparison to DN group. Here blood glucose level of DN
group was increased in comparison to normal control group while decreased significantly in extract treated group
at 14th, 21st, and 28th days in comparison to DN group. Conclusion: The overall results of this study have clearly
indicated that extract treated in a dose of 200 and 400 mg/kg ameliorate the renal ischemia reperfusion induced
nephropathy in diabetic rats. The action of methanolic extract contributed by renoprotective, anti-diabetic, and
antioxidant properties of C. trilocularis Linn.