Anti‑hypercholesterolaemic activity of Lipovedic and its mechanism
DOI:
https://doi.org/10.22377/ijgp.v7i2.315Abstract
Objective: To illustrate the antihypercholesterolaemic mechanism of Lipovedic ‑ a polyherbal formulation. Materials and Methods: The antihypercholesterolaemic mechanism of Lipovedic (180 mg/kg, p.o) was studied by measuring the serum total cholesterol (TC), triglycerides (TG), high density lipoprotein‑cholesterol (HDL‑C), low density lipoprotein‑cholesterol (LDL‑C), very low densitylipoprotein‑cholesterol (VLDL‑C), Faecal cholesterol (FC) and bile acid excretion in triton induced hyperlipidaemic rats at 0 h, 18 h,
24 h, 40 h, and 48 h and in high cholesterol diet (HCD) induced hypercholesterolaemic rats on day 7, 14, 21 and 28. Hydroxy‑3‑Methyl Glutaryl Co‑Enzyme A (HMG CoA)/mevalonate ratio was measured in the liver homogenate for the HMG‑CoA reductase activity. Atorvastatin (7.2 mg/kg p.o) was used as reference standard. One‑way ANOVA, Post‑hock analysis by Tukey’s multiple comparison tests was done using GraphPad Prism 5. Results: Lipovedic (180 mg/kg, p.o) reduced the serum TC, TG, LDL‑C, VLDL‑C and increased the serum HDL‑C in triton treated and HCD fed rats. Lipovedic increased FC excretion. HMG CoA/Mevalonate ratio was significantly higher compared to vehicle control rats and the result was comparable with Atorvastatin (7.2 mg/kg p.o). Conclusion:
Lipovedic acts as an anti‑hypercholesterolaemic drug by: inhibition of HMG CoA reductase, increasing FC excretion and reducing dietary cholesterol absorption, increasing hepatic low density lipoprotein receptor expression and by activation of Lecithin Cholesterol Acyl Transferase and Lipoprotein lipase.
Key words: High cholesterol diet, homogenate Co‑Enzyme A reductase, hypercholesterolemia, Lipovedic, triton, carboxy methyl
cellulose
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References
Dhulasavant V, Shinde S, Pawar M, Naikwade NS.
Antihyperlipidemic activity of Cinnamomum tamala Nees. on
high cholesterol diet inducedhyperlipidemia. Int J Pharm Tech
Res 2010;2:2517‑21.
Subramaniam S, Subramaniam R, Rajapandian S, Uthrapathi S,
Gnanamanickam VR, Dubey GP. Anti‑Atherogenic
Activity of Ethanolic Fraction of Terminalia arjuna Bark on
Hypercholesterolemic Rabbits. Evid Based Complement Alternat
Med 2011;2011:487916.
Sharma HL, Sharma KK. Principles of Pharmacology. Hyderabad:
Paras Medical Publisher; 2007. p. 72-75
Israni AD, Patel VK, Gandhi RT. Anti‑hyperlipidemic activity
of aqueous extract of Terminalia chebula and gaumutra in high
cholesterol diet fed rats. Pharma Sci monitor Int J Pharm Sci
;1:48‑59.
Suresh BS. Investigation of anti-obesity activity of a polyherbal
formulation in rats. Karnataka, India: Rajiv Gandhi University of
Health Sciences; 2004.
Ansarullah Jadeja RN, Thounaojam MC, Patel V, Devkar RV,
Ramachandra AV. Antihyperlipidaemic potential of a
polyherbal preparation on Triton WR 1339 (tyloxapol) induced
hyperlipidaemia: A comparison with Lovastatin. Int J Green Pharm
;3:119‑24.
Bharathi KN, Shankar DE. Evaluation of antioxidant and
hypolipidaemic activity of Vedic Guard, a polyherbal formulation.
Int J Pharma Sci Res 2010;1:114‑27.
Shastry MV. Comparitive evaluation of hypocholesterolemic
activity of Zingiber officinale extracts. Karnataka, India: Rajiv
Gandhi University of Health Sciences; 2009.
Total serum cholesterol. Available from: http://www.
sekisuidiagnostics.com/resources/PI/Total%20Serum%20
Cholesterol.pdf.[cited 2011 Feb 26].
Fossati P, Prencipe L. Serum triglycerides determined
colorimetrically with an enzyme that produces hydrogen peroxide.
Clin Chem 1982;28:2077‑80.
Burstein M, Scholnick HR, Morfin R. Rapid method for the
isolation of lipoproteins from human serum by precipitation with
polyanions. J Lipid Res 1970;11:583‑95.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the
concentration of low‑density lipoprotein cholesterol in plasma,
without use of the preparative ultracentrifuge. Clin Chem
;18:499‑502.
Vogel G, Vogel WH. Drug discovery and evaluation–
pharmacological assays. 2nd ed. Germany: Springer‑Verlag,
Berlin: Heidelberg; 2002. p. 1096.
Mosbach. Archive of biochem and biophysics. 1954;51: 402‑09.
Lee MJ, Crook T, Noel C, Levinson UM. Detergent extraction and
enzymatic analysis for fecal long‑chain fatty acids, triglycerides,
and cholesterol. Clin Chem 1994;40:2230‑4.
Rao AV, Ramakrishnan S. Indirect assessment of
hydroxymethylglutaryl‑CoA reductase (NADPH) activity in
liver tissue. Clin Chem 1975;21:1523‑5.
Jain PG, Patil SD, Haswani NG, Girase MV, Surana SJ.
Hypolipidemic activity of Moringa oleifera Lam., Moringaceae,
on high fat diet induced hyperlipidemia in albino rats. Rev Bras
Farmacogn Braz J Pharmacogn 2010;20:969‑73.
Jeyabalan S, Palayan M. Antihyperlipidemic activity of Sapindus
emarginatus in triton WR‑1339 induced albino rats. Res J Pharma
Tech 2009;2:319‑21.
Ghule BV, Ghante MH, Saoji AN, Yeole PG. Hypolipidaemic
and antihyperlipidaemic effects of Lagenaria siceraria (Mol.) fruit
extracts. Indian J Exp Biol 2006;44:905‑9.
Purnima AL, Mathuram V. Hypocholesterolaemic and
antihypercholesterolaemic activity of extracts of Trichilla cannaroids
on rats. Indian J Pharm Sci 2003;65:537‑9.
Maruthappan V, Sakthi Shree K. Antihyperlipidemic potential
of a polyherbal drug (Geriforte) on atherogenic diet induced
hyperlipidemia: A Comparison with Ayurslim. Int J Chem Analy
Sci 2010;1:37‑9.
Keshetty V, Pabba S, Gudipati R, Kandukuri JM, Allenki V.
Antihyperlipidemic activity of methanolic extract of Garlic (Allium
sativum L.) in triton X‑100 induced hyperlipidemic rats. J Pharma
Res 2009;2:777‑80.
Yazdanparast R, Bahramikia S. Evaluation of the effect of
Anethum graveolens L. crude extracts on serum lipids and
lipoproteins profiles in hypercholesterolaemic rats. DARU
;16:88‑94.
Kumarappan CT, Nageswara Rao T, Mandal SC. Polyphenolic
extract of Ichnocarpus frutescens modifies hyperlipidemia status
in diabetic rats. J Cell Mol Biol 2007;6:175‑87.
