Studies on the therapeutic potentials of Datura metel L. unripen fruits

Abstract

Introduction: Datura metel L. is a medicinal plant whose leaves and seeds are widely used in herbal medicine as anaesthetic, antispasmodic, antitussive, bronchodilator and as hallucinogenic. Materials and Methods how much does tramadol cost: Qualitative phytochemical analysis was performed to detect the presence of phytochemical constituents in a formulated Umaththankai parpam using a common plant Datura metel L with Ocimum sanctum leaves and sulphur. Antibacterial and antifungal analysis were carried out to determine its therapeutic potentials. The phytochemical compounds present in Umaththankai parpam was identified by GC-MS analysis with their molecular weight (MW), retention time (RT), concentration (peak area %), Molecular formula (MF). Results: The qualitative phytochemical analysis revealed the presence of various secondary metabolites and found to be containing alkaloids, flavonoids, saponins, fixed oils, tannin, phenols and glycosides; while protein, amino acid, steroids and terpenoids were found to be absent. The data showed that alkaloids, glycosides and flavonoids were found as strong positive than other metabolites. The crude extract has antibacterial activity against the Gram negative bacteria such as Pseudomonas aeruginosa and Escherichia coli and the Gram positive bacteria Staphylococcus aureus and also the phytochemicals exhibited antifungal activity against three fungal species such as Candida albicans, Aspergillus niger which are causing secondary infections to COVID19 patients and Tricophyton rubrum. Totally 10 compounds were identified and the high peak area compounds in this sample cost of tramadol were n- Hexadececanoic acid (54.24%) and others were 9,12- Octadecadienoyl chloride (15.63%), 9,12- Octadecadienoic acid(12.97%), Z,E-2-Methyl-3,13-octadecadien-1-ol(12.96%), Hexadecanoic acid, 1-(hydroxymethyl)-1,2-ethanediyl ester (2.30) and the rest of the compounds showing short peak area like 6-Tetradecanol(0.71%), 9,12-Octadecadien-1-ol, (Z,Z)- (0.54%), Benzene, 1,2-dimethoxy-4-(1-propenyl)-(0.52%), Methyl 6-methyl heptanoate (0.07%) and (S)-3,4-Dimethylpentanol (0.06%). Discussion and Conclusions: The peak compound n-hexadecanoic acid is a known anti-inflammatory agent and which was well documented in seaweeds but surprisingly the same compound has been found in our novel formulation. The second peak compound was 9,12-octadecadienoic acid and which has been reported as an antimicrobial compound in a different source. The study has brought out a wonderful formulation with readily available plant resources with potential therapeutic compounds and proved to be a drug source.