Development and validation of a simple and precise analytical technique for the simultaneous quantification of drospirenone and estetrol in bulk and tablets using RP-HPLC

Abstract

Aim: For the simultaneous estimate of drospirenone (DSP) and estetrol (ESR), a new oral contraceptive in bulk
and dose forms, a simple methodology was established using RP-HPLC. Methods: Analytes were separated
using Methanol: Phosphate Buffer pH 6.8 adjusted with dil. NaOH (40:60, v/v) as mobile phase pumped at
1.0 ml/min on a Waters C18 Column with 250 mm 4.6 mm i.d. and 5 m particle size.