Hepatoprotective effect of hydroalcoholic extract of Qurse Afsanteen, apolyherbal Unani formulation, inchemically induced hepatotoxicity in rat

Abstract

Aim: The liver plays a key role in the biotransformation of many chemicals and drugs. Thereby, it is a target organ
for several toxicants. Qurse Afsanteen, an Unani formulation, is claimed to be effective as a hepatoprotective.
Materials and Methods: To evaluate the hepatoprotective effect of hydroalcoholic extract of Qurse Afsanteen
(HAEQA) against CCl4 and Rifampicin induced hepatotoxicity, 36 rats were divided into six groups, each having six
animals representing negative, positive, standard, and test Groups A, B, and C. CCl4 was used in the dose of 0.7 ml/
kg for 7 days whereas Rifampicin was given in the dose of 100 mg/kg for 15 days for inducing hepatotoxicity. In
test and standard groups, CCl4 and Rifampicin were administered after 30 min of receiving test and standard drug.
Treatment Groups A, B, and C received HAEQA in three doses, that is, 40.32 mg, 67.2 mg, and 120.96 mg/kg
to observe the dose-dependent effect. Silymarin (100mg/kg) was used as standard drug. Serum markers, namely,
SGOT, SGPT, ALP, bilirubin, and cholesterol were estimated as parameter. Histopathology of the liver was also
done. Result and Discussion: HAEQA showed significant hepatoprotection at therapeutic dose level (67.2 mg/
kg) against CCl4 and Rifampicin-induced hepatotoxicity which is evident by decreased enzyme markers in both
methods. Conclusion: The findings were suggestive of the test drug possessing hepatoprotective activity.