Anti‑inflammatory effect of hydromethanolic extract from Marrubium vulgare Lamiaceae on leukocytes oxidative metabolism: An in vitro and in vivo studies
DOI:
https://doi.org/10.22377/ijgp.v7i3.327Abstract
Background: Marrubium vulgare Lamiaceae, known as white horehound, is frequently used in folk medicine to treat many illnesses from ancient times. Aims: The present study is conducted to evaluate the anti‑inflammatory activity of hydromethanolic extract of the aerial part of M. vulgare. Materials and Methods: The oxygen consumption and the production of superoxide anions, by rat
pleural polymorphonuclear leukocytes stimulated by opsonized zymosan, were performed. PMNs were collected after induction
of an acute inflammatory reaction by injection, in the rat pleural cavity, of suspension of calcium pyrophosphate crystals. Plant
extract was tested at a concentration range 10‑100 mg/ml and 100‑400 mg/kg/day for in vitro and in vivo assays respectively.
Statistical analysis: All values are expressed as mean ± standard error. The significance of differences between control and treated
samples in vitro was analysed using the Student’s t‑test. For in vivo studies, data were analysed using one‑way analysis of variance. Differences with P ≤ 0.05 were considered to be statistically significant. Results: Hydromethanolic extract of M. vulgare showed, in vitro a significant anti‑inflammatory effect in a dose‑dependent manner. In vivo, only the high amounts of the plant extract (300 and 400 mg/kg/day) exhibited a significant anti‑inflammatory effect. The plant extract contains a high amount of polyphenols.
Conclusion: Hydromethanolic extract of M. vulgare had an anti‑inflammatory effect, which corroborates the usefulness of this plant in the treatment of inflammatory disorders.
Key words: Anti‑inflammatory effect, Marrubium vulgare, oxygen consumption, polymorphonuclear leukocytes, polyphenols,
superoxide anions generation
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References
Fantone JC, Ward PA. Role of oxygen‑derived free radicals and
metabolites in leukocyte‑dependent inflammatory reactions. Am
J Pathol 1982;107:395‑418.
Roos D. The respiratory burst of phagocytic leukocytes. Drug
Investig 1991;3:48‑53.
Pryor WA. Cancer and free radicals. Basic Life Sci 1986;39:45‑59.
Pryor WA. FORUM: Oxidation and atherosclerosis Free Radic Biol
Med 2000;28:1681‑2.
Bellakhdar J. La Pharmacopée Marocaine Traditionnelle. France:
Ibis Press editor; 1997.
El Abbouyi A, Toumi M, El Hachimi Y, Jossang A. In vitro
effects of aqueous seeds extract of Acacia cyanophylla on the
opsonized zymosan‑induced superoxide anions production by rat
polymorphonuclear leukocytes. J Ethnopharmacol 2004;91:159‑65.
Willoughby DA, Dunn CJ, Yamamoto S, Capasso F, Deporter DA,
Giroud JP. Calcium pyrophosphate‑induced pleurisy in rats:
A new model of acute inflammation. Agents Actions 1975;5:35‑8.
Paul JL, el Abbouyi A, Roch‑Arveiller M, Giroud JP, Agneray J,
Yonger J, et al. Effects of storage on the pyruvate‑lactate system
and random migration of human granulocytes. Vox Sang
;52:24‑6.
Johnston RB Jr, Keele BB Jr, Misra HP, Lehmeyer JE, Webb LS,
Baehner RL, et al. The role of superoxide anion generation in
phagocytic bactericidal activity. Studies with normal and chronic
granulomatous disease leukocytes. J Clin Invest 1975;55:1357‑72.
Kvarstein B, Halvorsen KS. Oxygen consumption during the
initial stage of human leucocyte phagocytosis as a test for chronic
granulomatous disease. Scand J Clin Lab Invest 1970;26:175‑8.
Singleton VL, Joseph A, Rossi JR. Colorimetry of total phenolics
with phosphomolybdic‑phosphotungstic acid reagents. Am J Enol
Viticulture 1965;16:144‑53.
Kanyonga PM, Faouzi MA, Meddah B, Mpona1 M, Essassi EM,
Cherrah Y. Assessment of methanolic extract of Marrubium vulgare
for antiinflammatory, analgesic and anti‑microbiologic activities.
J Chem Pharm Res 2011;3:199‑204.
Nicholas HJ. Isolation of marrubin, a sterol, and a sesquiterpene
from marrubium vulgare. J Pharm Sci 1964;53:895‑9.
Knöss W, Reuter B, Zapp J. Biosynthesis of the labdane diterpene
marrubiin in Marrubium vulgare via a non‑mevalonate pathway.
Biochem J 1997;326 (Pt 2):449‑54.
Wojdyło A, Oszmianski J, Czemerys R. Antioxidant activity
and phenolic compounds in 32 selected herbs. Food Chem
;105:940‑9.
Kadri A, Zarai Z, Békir A, Gharsallah N, Damak M, Gdoura R.
Chemical composition and antioxidant activity of Marrubium
vulgare L essential oil from Tunisia. Afr J Biotechnol 2011;10:3908‑14.
Boudjelal A, Henchiri C, Siracusa L, Sari M, Ruberto G.
Compositional analysis and in vivo anti‑diabetic activity of wild
Algerian Marrubium vulgare L. infusion. Fitoterapia 2012;83:286‑92.
Zawislak G. The chemical composition of the essential oil of
Marrubium vulgare L from Poland. Farmacia 2012;60:287‑92.
Grassmann J, Hippeli S, Elstner EF. Plant’s defense and its benefits
for animals and medicine: role of phenolics and terpenoids in
avoiding oxygen stress. Plant Physiol Biochem 2002;40:471‑8.
Kanashiro A, Kabeya LM, Polizello AC, Lopes NP, Lopes JL,
Lucisano‑Valim YM. Inhibitory activity of flavonoids from
Lychnophora sp. on generation of reactive oxygen species by
neutrophils upon stimulation by immune complexes. Phytother
Res 2004;18:61‑5.
Yoon JH, Baek SJ. Molecular targets of dietary polyphenols with
anti‑inflammatory properties. Yonsei Med J 2005;46:585‑96.
Busse WW, Kopp DE, Middleton E Jr. Flavonoid modulation of
human neutrophil function. J Allergy Clin Immunol 1984;73:801‑9.
Takemura OS, Banno Y, Nozawa Y. Inhibition of
N‑formylmethionyl‑leucyl‑phenylalanine‑stimulated tyrosine
phosphorylation and phospholipase D activation by quercetin in
rabbit neutrophils. Biochem Pharmacol 1997;53:1503‑10.
Stulzer HK, Tagliari MP, Zampirolo JA, Cechinel‑Filho V,
Schlemper V. Antioedematogenic effect of marrubiin obtained
from Marrubium vulgare. J Ethnopharmacol 2006;108:379‑84.
Tauber AI, Fay JR, Marletta MA. Flavonoid inhibition of the human
neutrophil NADPH‑oxidase. Biochem Pharmacol 1984;33:1367‑9.
Visioli F, Bellomo G, Galli C. Free radical‑scavenging properties of
olive oil polyphenols. Biochem Biophys Res Commun 1998;247:60‑4.
