Effect of aqueous enriched fraction of Premna integrifolia root against cafeteria diet induced obesity in Swiss albino mice
DOI:
https://doi.org/10.22377/ijgp.v7i4.341Abstract
Objective: The objective of the present study was to evaluate the effect of aqueous enriched fraction of Premna integrifoliaroot (AEFPIR) against cafeteria diet induced obesity in Swiss albino mice. Materials and Methods: Female Swiss albino mice were divided into four groups, which received cafeteria diet, standard drug simvastatin (10 mg/kg) and AEFPIR (200 and 400 mg/kg) daily for 40 days. The body weight, body mass index (BMI), food consumption, serum glucose, triglyceride, total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL) were studied along with histopathological assessments for screening the effect of AEFPIR against cafeteria diet induced obesity. High performance liquid chromatography (HPLC) fingerprint profile of AEFPIR was also studied using quercetin as the reference standard. Results: The results of present study revealed
that, there was a significant decrease in body weight, BMI, food consumption and in the levels of serum glucose, triglyceride, total cholesterol, LDL and VLDL with a significant increase in the level of HDL in mice treated with simvastatin and AEFPIR groups
compared with cafeteria diet group. Mice treated with AEFPIR shows dose dependent effect. The AEFPIR (400 mg/kg) supplementation attenuated all the above alterations, which indicates the protective effect against cafeteria diet induced obesity that was further confirmed by histopathological analysis. The solvent system was used for HPLC fingerprint profile of AEFPIR, 50 Mm potassium diphosphate (pH‑3 with ortho phosphoric acid): Methanol (50:50 v/v) at 360 nm. The chromatograph showed three peaks at retention
times 3.835, 5.649 and 11.106. Conclusion: The present study suggests that AEFPIR possess protective effect against cafeteria diet induced obesity that was substantiated its ethno‑medicinal use in the treatment of obesity. The exploration of chemical constituents and further pharmacological evaluation will give us basis for its therapeutic use. Further series of studies are required to prove its clinical reliability, safety and efficacy.
Key words: Aqueous enriched fraction, cafeteria diet, obesity, Premna integrifolia
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References
Chunekar KC. Illustrated Dravyaguna Vijnana. 2nd ed. Vol. 2.
Varanasi: Chaukhambha Orientalia; 2005. p. 418‑21.
Warrier PK, Nambiar VP. Indian Medicinal Plants. Madras: Orient
Longman; 1997. p. 348‑52.
Mali PY, Bigoniya P, Panchal SS, Muchhandi IS. Anti‑obesity
activity of chloroform‑methanol extract of Premna integrifolia in
mice fed with cafeteria diet. J Pharm Bioallied Sci 2013;5:229‑36.
George KV, Samuel KA, Abraham J, George BP. Investigations
on bioactive compounds of Premna integrifolia Linn, 2008. [About
pages]. Available from: http://www.forestry.caf.wvu.edu/
wvu_woodscience/bdawson/web_bibu/Premna.pdf. [Last cited
on 2008 Feb 20].
Basu NK, Dandiya PC. Chemical investigation of Premna integrifolia
Linn. J Am Pharm Assoc Am Pharm Assoc 1947;36:389‑91.
Basu NK, Joneja AN. Chemical investigation of Premna integrifolia.
Indian J Pharm 1949;11:191‑3.
Barik BR, Bhowmik AK, Dey AK, Patra A, Chatterjee A, Joy S,
et al. Premnazole, an isoxazole alkaloid of Premna integrifolia
and Gmelina arborea with anti‑inflammatory activity. Fitoterapia
;63:295‑9.
Gokani RH, Kapadiya NS, Shah MB. Comparative pharmacognostic
study of Clerodendrum phlomidis and Premna integrifolia. J Nat
Remed 2008;8:222‑31.
Gokani RH, Shah MB. Isolation and estimation of Clerodendrum
phlomidis and Premna integrifolia root. J Pharm Res 2009;8:9‑11.
Singh CR, Nelson P. Muthu K, Pargavi B. Identification of volatile
constituents from Premna serratifolia L. through GC‑MS. Int J Pharm
Tech Res 2011;3:1050‑8.
Singh CR, Nelson P, Boopathy S. In‑vitro conservation and
protective effect of Premna serratifolia Linn.: An important
medicinal tree. Int J Pharm Appl 2012;3:332‑43.
Yadav D, Tiwari N, Gupta MM. Diterpenoids from Premna
integrifolia. Phytochem Lett 2010;3:143‑7.
Yadav D, Tiwari N, Gupta MM. Simultaneous quantification
of diterpenoids in Premna integrifolia using a validated HPTLC
method. J Sep Sci 2011;34:286‑91.
Bose LV, Varghese GK, Habtemariam S. Identification of acteoside
as the active antioxidant principle of Premna serratifolia root wood
tissues. Phytopharmacology 2013;4:228‑36.
Mali PY, Bhadane VV. Comparative account of screening of
bioactive ingredients of Premna integrifolia Linn. with special
reference to root by using various solvents. J Pharm Res
;3:1677‑9.
Alamgir M, Rokeya B, Hannan JM, Choudhuri MS. The effect
of Premna integrifolia Linn. (Verbenaceae) on blood glucose in
streptozotocin induced type 1 and type 2 diabetic rats. Pharmazie
;56:903‑4.
Kar A, Choudhary BK, Bandyopadhyay NG. Comparative
evaluation of hypoglycaemic activity of some Indian medicinal
plants in alloxan diabetic rats. J Ethnopharmacol 2003;84:105‑8.
Dash GK, Patro CP, Maiti AK. A study on the antihyperglycaemic
effect of Premna corymbosa Rottl. roots. J Nat Remed 2005;5:31‑4.
Rathore RS, Prakash A, Singh PP. Preliminary study of
anti‑inflammatory and anti‑arthritic activity. Rheumatism
;12:130.
Sasidharan N. Qualitative and quantitative analysis of biologically
active principles, baicalein, luteolin and psoralen from Oroxylum
indicum, Premna serratifolia, Aegle marmelos and their allied species.
KFRI Research Report No. 350 (KFRI/486/05). Peechi: Kerala Forest
Research Institute; 2010. p. 1‑57.
Gokani RH, Lahiri SK, Santani DD, Shah MB. Evaluation of
immunomodulatory activity of Clerodendrum phlomidis and Premna
integrifolia root. Int J Pharmacol 2007;3:352‑6.
Rajendran R, Suseela L, Meenakshi SR, Saleem BN. Cardiac
stimulant activity of bark and wood of Premna integrifolia.
Bangladesh J Pharmacol 2008;3:107‑13.
Rajendran R, Basha NS. Cardioprotective effect of ethanol extract of
stem‑bark and stem‑wood of Premna serratifolia Linn. (Verbenaceae).
Res J Pharm Technol 2008;1:487‑91.
Kumar KU, Soma P, Kumar SS, Chandra SM, Kumar BS.
Assessment of analgesic and antibacterial activity of Premna
integrifolia Linn. (Family: Verbenaceae) leaves. Int J Pharm Sci Res
;2:1430‑5.
Singh CR. Antimicrobial effect of callus and natural plant extracts
of Premna serratifolia L. Int J Pharma Biomed Res 2011;2:17‑20.
Rajendran R, Basha NS. Antimicrobial activity of crude extracts and
fractions of Premna serratifolia Linn. root. Med Plants 2010;2:33‑8.
Rajendran R. Antimicrobial activity of different bark and wood of
Premna serratifolia Linn. Int J Pharma Biosci 2010;6:1‑9.
Rajendran R, Krishnakumar E. Anti‑arthritic activity of Premna
serratifolia Linn., wood against adjuvant induced arthritis.
Avicenna J Med Biotechnol 2010;2:101‑6.
Singh CR, Nelson R, Krishnan PM, Mahesh K. Hepatoprotective
and anti‑oxidant effect of root and root callus extract of Premna
serratifolia L. in paracetamol induced liver damage in male albino
rats. Int J Pharma Biosci 2011;2:244‑52.
Selvam NT, Vengatakrishnan V, Damodar KS, Murugesan S.
Evaluation of tissue level antioxidant activity of Premna serratifolia
leaf in paracetamol intoxicated Wistar albino rats. Int J Pharm Life
Sci 2010;1:86‑90.
Vadivu R, Jerad S, Girinath K, Kannan PB, Vimala R, Kumar NM.
Evaluation of hepatoprotective and in‑vitro cytotoxic activity of
leaves of Premna serratifolia Linn. J Sci Res 2009;1:145‑52.
Selvam TN, Venkatakrishnan V, Damodar KS, Elumalai P.
Antioxidant and tumor cell suppression potential of Premna
serratifolia linn leaf. Toxicol Int 2012;19:31‑4.
Bagchi C, Tripathi SK, Hazra A, Bhattacharya D. Evaluation of
hypolipidemic activity of Premna integrifolia Linn. Bark in rabbit
model. Pharmbit 2008;18:149‑53.
Karthikeyan M, Deepa MK. Anti‑hyperlipidemic activity of Premna
corymbosa (Burm.f.) Rottl. and Willd. in liver damaged Wistar
albino rats. J Pharm Res 2008;1:61‑4.
Patel MJ, Patel JK, Evaluation of the anti‑hyperlipidemic activity
of Premna integrifolia using experimental animal model. Int J Res
Phytochem Pharmacol 2011;1:146‑9.
Patel MJ, Patel JK. Evaluation of the anti‑hyperlipidaemic activity
of Premna integrifolia on nicotine induced hyperlipidaemia in rats.
Int J Pharma Biosci 2012;3:226‑32.
Banu UK. Evaluation of neuroprotective effect in the experimental
model of febrile seizure of the roots of Premna serratifolia Linn.
International Herbal Conference. Banglore, 2009. (Poster No. SNPS
IHCB‑09781). Available from: http://www.herbalconference2009.
com. [Last cited on 2013 Aug 29].
Desrivot J, Waikedre J, Cabalion P, Herrenknecht C, Bories C,
Hocquemiller R, et al. Antiparasitic activity of some New
Caledonian medicinal plants. J Ethnopharmacol 2007;112:7‑12.
Yadav D, Masood N, Luqman S, Brindha P, Gupta MM.
Antioxidant furofuran lignans from Premna integrifolia. Industrial
Crops Prod 2013;41:397‑02.
Gokani RH, Lahiri SK, Santani DD, Shah MB. Evaluation of
in‑vitro anti‑oxidant activity of Premna integrifolia Linn. root. Res
J Pharmacog Phytochem 2010;2:196‑9.
Gopal RH, Purushothaman KK. Effects of plant isolates on
coagulation of blood: An in‑vitro study. Bull Med Ethnobot Res
;5:171‑7.
Sridharan G, Brindha P, Jaiganesh C, Sivasubramanian S. Anti
tumor potential of Premna integrifolia Linn. against Ehrlich ascites
carcinoma cell lines. Pharmacologyonline 2011;2:438‑50.
Jothi ET, Karthikeyan R, Suryalakshmi PV, Srinivasababu P.
Gastroprotective potential of Premna serratifolia Linn. leaves
against aspirin induced ulcer in albino rats. Pharmacologyonline
;3:189‑98.
Shukla V, Yadav D, Phulara SC, Gupta MM, Saikia SK, Pandey R.
Longevity‑promoting effects of 4‑hydroxy‑E‑globularinin in
Caenorhabditis elegans. Free Radic Biol Med 2012;53:1848‑56.
Parle M, Vasudevan M. Memory enhancing activity of Abana: An
Indian ayurvedic poly herbal formulation. J Health Sci 2007;53:43‑52.
Jabbar S, Khan MT, Choudhuri MS, Sil BK. Bioactivity studies of
the individual ingredients of the Dashamularishta. Pak J Pharm Sci
;17:9‑17.
Khanna AK, Chander R, Kapoor NK. Hypolipidaemic activity of
Abana in rats. Fitoterapia 1991;3:271.
Flier JS. Obesity wars: Molecular progress confronts an expanding
epidemic. Cell 2004;116:337‑50.
Haslam DW, James WP. Obesity. Lancet 2005;366:1197‑209.
Hall JE, Crook ED, Jones DW, Wofford MR, Dubbert PM.
Mechanisms of obesity‑associated cardiovascular and renal
disease. Am J Med Sci 2002;324:127‑37.
Sowers JR. Update on the cardiometabolic syndrome. Clin
Cornerstone 2001;4:17‑23.
World Health Organization. Obesity: Preventing and managing
the global epidemic. Report of a WHO Consultation on Obesity.
Geneva: WHO; 1998.
Nammi S, Koka S, Chinnala KM, Boini KM. Obesity: An overview
on its current perspectives and treatment options. Nutr J 2004;3:3.
Jindal V, Dhingra D, Sharma S, Parle M, Harna RK. Hypolipidemic
and weight reducing activity of the ethanolic extract of Tamarindus
indica fruit pulp in cafeteria diet‑ and sulpiride‑induced obese rats.
J Pharmacol Pharmacother 2011;2:80‑4.
Flegal KM, Graubard BI, Williamson DF, Gail MH. Cause‑specific
excess deaths associated with underweight, overweight, and
obesity. JAMA 2007;298:2028‑37.
Eckel RH. Clinical practice. Nonsurgical management of obesity
in adults. N Engl J Med 2008;358:1941‑50.
Phani KG, Kumar R, Badere R, Singh SB. Antibacterial and
antioxidant activities of ethanol extracts from trans Himalayan
medicinal plants. Pharmacog J 2010;2:66‑9.
Organization for Economic Co‑operation and Development (OECD)
Guideline for Testing of Chemicals. Acute oral toxicity‑Up and
down procedure. OECD Test Guideline No. 425., 2008. p. 1‑27.
Available from, http://iccvam.niehs.nih.gov/SuppDocs/FedDocs/
OECD/OECDtg425.pdf. [Last cited on 2013 Sep 10].
Organization for Economic Co‑operation and Development (OECD)
Guideline for Testing of Chemicals. Acute Oral Toxicity – Fixed
Dose Procedure. OECD Test Guideline No. 420. 2001. p. 1‑14.
Available from, http://iccvam.niehs.nih.gov/SuppDocs/FedDocs/
OECD/OECD_GL420.pdf. [Last cited on 2013 Sep 10].
Kaur G, Kulkarni SK. Anti‑obesity effect of a polyherbal
formulation, OB200G in female rats fed on cafeteria and
atherogenic diets. Indian J Pharmacol 2000;32:294‑9.
Harris RB. The impact of high‑ or low‑fat cafeteria foods on
nutrient intake and growth of rats consuming a diet containing
% energy as fat. Int J Obes Relat Metab Disord 1993;17:307‑15.
Gallou‑Kabani C, Vigé A, Gross MS, Boileau C, Rabes JP,
Fruchart‑Najib J, et al. Resistance to high‑fat diet in the female
progeny of obese mice fed a control diet during the periconceptual,
gestation, and lactation periods. Am J Physiol Endocrinol Metab
;292:E1095‑100.
Auzi AR, Hawisa NT, Sherif FM, Sarker SD. Neuropharmacological
properties of Launaea resedifolia. Braz J Pharmacol 2007;17:160‑5.
Basarkar PW, Nath N. Hypocholesterolemic and hypolipidemic
activity of quercetin: A vitamin P‑like compound in rats. Indian J
Med Res 1983;77:122‑6.
Sclafani A, Springer D. Dietary obesity in adult rats: Similarities
to hypothalamic and human obesity syndromes. Physiol Behav
;17:461‑71.
Bull NL. Studies of the dietary habits, food consumption and
nutrient intakes of adolescents and young adults. World Rev Nutr
Diet 1988;57:24‑74.
Rothwell NJ, Stock MJ, Warwick BP. The effect of high fat and high
carbohydrate cafeteria diets on diet‑induced thermogenesis in the
rat. Int J Obes 1983;7:263‑70.
Naim M, Brand JG, Kare MR, Carpenter RG. Energy intake,
weight gain and fat deposition in rats fed flavored, nutritionally
controlled diets in a multichoice (“cafeteriaâ€) design. J Nutr
;115:1447‑58.
Barr HG, McCraken KJ. High efficiency of energy utilization
in “cafeteria-“ and force‑fed rats kept at 29 degrees. Br J Nutr
;51:379‑87.
Boden G. Role of fatty acids in the pathogenesis of insulin
resistance and NIDDM. Diabetes 1997;46:3‑10.
Hotamisligil GS, Shargill NS, Spiegelman BM. Adipose expression
of tumor necrosis factor‑alpha: Direct role in obesity‑linked insulin
resistance. Science 1993;259:87‑91.
Grundy SM, Barnett JP. Metabolic and health complications of
obesity. Dis Mon 1990;36:641‑731.
Campillo JE, Torres MD, Dominguez E, Romeroa A, Perrez C.
Ficus carica lead administration reduces hypertriglyceridemia in
streptozotocin diabetic rats. Diabetologia 1994;37:213.
Byers SO, Fiedman M, Sugiyama T. Triton hypercholesteremia:
Cause or consequence of augmented cholesterol synthesis. Am J
Physiol 1963;204:1100‑2.
Warnholtz A, Mollnau H, Oelze M, Wendt M, Münzel T.
Antioxidants and endothelial dysfunction in hyperlipidemia. Curr
Hypertens Rep 2001;3:53‑60.
Muthu AK, Sethupathy S, Manavalan R, Karar PK. Effect of
methanolic extract of Dolichos biflorus Linn. on lipid profile of
rabbits fed with high fat diet. Adv Pharmacol Toxicol 2007;8:19‑24.
Tenpe CR, Thakare AB, Upaganlawar AB, Yeole PG. Hypolipidemic
and weight‑controlling activity of Terminalia catappa Linn. in rats
on sucrose‑high fat diet. Indian Drugs 2007;44:16‑20.
Howell TJ, MacDougall DE, Jones PJ. Phytosterols partially explain
differences in cholesterol metabolism caused by corn or olive oil
feeding. J Lipid Res 1998;39:892‑900.
Lemhadri A, Hajji L, Michel JB, Eddouks M. Cholesterol and
triglycerides lowering activities of caraway fruits in normal and
streptozotocin diabetic rats. J Ethnopharmacol 2006;106:321‑6.
Johns T, Chapman LI, Amason JT, Mata R, Romeo JT. Phytochemistry
of Medicinal Plants. 3rd ed. New York: Kluwer Academic
Publishers; 1995.
Tan MH, Johns D, Glazer NB. Pioglitazone reduces atherogenic
index of plasma in patients with type 2 diabetes. Clin Chem
;50:1184‑8.
