In vitro antimicrobial activities of anthrones from the leaf latex of Aloe sinana Reynolds
DOI:
https://doi.org/10.22377/ijgp.v8i1.347Abstract
Background: Aloe sinana Reynolds is endemic to Ethiopia where its leaf latex is traditionally used in and around the town of Debre Sina and other central highlands of the country for the treatment of various illnesses, including wound, snake bite and malaria. However, despite its use in traditional medicine, to date, there appears to have been no chemical or biological studies published on this plant.Aim: The aim of this study was to investigate the leaf latex of A. sinana for its antibacterial and antifungal activities, and to isolate and characterise the compounds that are responsible for the antimicrobial effect of the latex. Materials and Methods: The latex was extracted with methanol. Isolation of compounds was achieved by repeated preparative TLC, and spectroscopic techniques including 1H, 13C‑NMR and MS were used for characterisation of the isolated compounds. Antimicrobial activity tests were performed against 21 bacterial and 4 fungal pathogens using the disc diffusion method. Results and Conclusion: Three compounds isolated
from the leaf latex were identified as the anthrones, aloin, aloinoside and microdontin. Among the isolated compounds, aloinoside and microdontin were found to possess comparable activity (MIC 5 μg/ml) with that of ciprofloxacin against several Gram‑negative bacterial strains and Staphylococcus aureus. Additionally, microdontin showed potent and comparable activity with the standard antifungal drug griseofulvin against Penicillum spp. These findings support the traditional uses of the plant for the treatment of various infections and wound.
Key words: Aloe sinana, anthrones, antibacterial, antifungal, disc diffusion, latex
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References
WHO 2012. World Malaria Report: Global report for research on
infectious diseases of poverty, Geneva: 2012.
Rawat V, Upadhyaya K. Evaluation of antimicrobial activity and
preliminary phytochemical screening of Mesua ferrea seeds extract.
J Nat Prod 2013;6:17‑26.
Khaing TA. Evaluation of the antifungal and antioxidant activities
of the leaf extract of Aloe vera. World Acad Sci Eng Technol
;75.
Mann A, Banso A, Clifford LC. An antifungal property of
crude plant extracts from Anogeissus leiocarpus and Terminalia
avicennioides. Tanzania J Health Res 2008;10:34‑8.
Kassaye KD, Amberbir A, Getachew B, Mussema Y. A historical
overview of traditional medicine practices and policy in Ethiopia.
Ethiop J Health Dev 2006;20:127‑34.
University of California Davis Botanical Conservatory (UCDAVIS).
The Genus Aloe Botanical notes. 2009:1:1‑11.
Bekele E. Study on actual situation of medicinal plants in Ethiopia.
Tokyo: Japan Association for International Collaboration of
Agriculture and Forestry; 2007. p. 20.
Demissew S, Nordal I. Aloes and Lilies of Ethiopia and Eritrea.
Addis Ababa: Shama Books, 2003. p. 42‑3.
Minale G. In vivo antimalarial and in vitro antimicrobial evaluation
of latex and compounds isolated from the leaf latex of Aloe
sinana Reynolds. M. Sc. Thesis. Addis Ababa: Addis Ababa
University;2013.
Institute of Laboratory Animal Resources: Guide for the care
and use of laboratory animals. 7th ed. Washington DC: National
Academy Press;1996.
OECD Guidelines, OECD guidelines for testing of chemicals, Test
No. 425, Acute toxic class method;2008.
Mitchell JK, Carter WE. Modeling antibacterial activity of CloroxTM
using agar‑diffusion test: A new twist on an old experiment. Biosci
;26:9‑13.
Hecht DW, Citron DM, Cox M, Jacobus N, Jenkins SG,
Onderdonk A, Roe‑Carpenter D, Rosenblatt JE, Wexler HM.
Methods for antimicrobial susceptibility testing of anaerobic
bacteria; approved standard‑seventh edition. Clinical and
Laboratory Standards Institute (CLSI). 2007. http://www.clsi.org/
source/orders/free/m11a7.pdf. [Last accessed on 2013 Jan 10].
Jayasinghe CD, Udagama‑Randeniya PV, Ratnasooriya WD.
In vivo antimalarial activity of aqueous root extract of Barringtonia
acutangula in mice. Pharmacogn Mag 2008;4:51‑8.
Dagne E, Alemu M. Constituents of the leaves of four Aloe species
from Ethiopia. Bull Chem Soc Ethiop 1991;5:87‑91.
Farah MH, Andersson R, Samuelsson G. Microdontin A and B:
Two new aloin derivatives from Aloe microdonta. Planta Medica
;58:88‑93.
Asamenew G, Bisrat D, Mazumder A, Asres K. In vitro
antimicrobial and antioxidant activities of anthrone and
chromone from the latex of Aloe harlana Reynolds. Phytother Res
;25:1756‑60.
O’Sullivan J, Bolton DJ, Duffy G, Baylis C, Tozzoli R,
Wasteson Y, Lofdahl S. Methods for detection and molecular
characterisation of pathogenic Escherichia coli. Co‑ordination
action FOOD‑CT‑2006‑036256. Pathogenic Escherichia coli network.
Editors O’Sullivan J, Bolton DJ, Duffy G, Baylis C, Tozzoli R,
Wasteson Y, Lofdahl S. 2007. http://www.antimicrobialresistance.
dk/data/images/protocols/e%20coli%20methods.pdf.
[Last accessed on 2013 Jan 10].
Wang J, Zhao H, Kong W, Jin C, Zhao Y, Qu Y, et al. Microcalorimetric
assay on the antimicrobial property of five hydroxyanthraquinone
derivetives in rhubarb (Rheum palmatum L) to Bifidobacterium
adolescentis. Phytomedicine 2010;17:684‑9.
Lu C, Wang H, Lu W, Xu P, Zhu J, Xie J, et al. Antibacterial
properties of anthraquinones extracted from rhubarb against
Aeromonas hydrophila. Fisheries Sci 2011;77:375‑84.
Wu Y-W, Ouyang J, Xiao X-H, Gao W-Y, Liu Y. Antimicrobial
properties and toxicity of anthraquinones by microcalorimetric
bioassay. Chin J Chem 2006;24:45‑50.
Wendakoon C, Calderon P, Gagnon D. Evaluation of selected
medicinal plants extracted in different ethanol concentrations
for antibacterial activity against human pathogens. J Med Active
Plants 2011;1:60‑8.
Kambizi L, Afolayan AJ. Extracts from Aloe ferox and Withania
somnifera inhibit Candida albicans and Neisseria gonorrhea. Afr J
Biotechnol 2008;7:12‑5.
