Immunostimulatory effect of standardised alcoholic extract of green tea (Camellia sinensis L.) against cyclophosphamide‑induced immunosuppression in murine model
DOI:
https://doi.org/10.22377/ijgp.v8i1.354Abstract
Backgrounds: Tea, Camellia sinensis (L.) O. Kuntze (Theaceae) is one of the most widely consumed beverages in the world. It can be classified into three major types, depending on the level of fermentation, that is, green and white (unfermented), oolong (partially fermented) and black (fermented) tea. Each type of tea has a distinct composition, dependent on how the leaves are processed, as well as maturation, geographical location and agricultural practices. Green tea (GT), the least processed tea, is one of the highest studied and is ascribed to have the highest content of phenolic compounds. Aim: The current study was intended to evaluate the immunostimulatory potential of alcoholic extract of green tea (GTEA or GTAE) in cyclophosphamide (CP)‑induced immunosuppression in murine model. Materials and Methods: GTEA was standardised by high pressure thin liquid chromatography (HPTLC). CP (50 mg/kg, i.p.) was used to induce immunosuppression in mice. GTAE (50, 150 and 250 mg/kg) was administered orally for 14 day. Results: GTEA (150 and 250 mg/kg) treatment significantly (P < 0.001) elevated the thymus and spleen weight, haemagglutination titre and total leucocyte counts level and lowered the delayed type hypersensitivity response as compared with CP‑induced immunosupressed mice.Conclusions: These observations suggest immunostimulatory effects of GTAE against CP‑induced immunosuppression in murine model.
Key words: Cyclophosphamide, delayed type hypersensitivity, green tea, Haemagglutination titre
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References
Ziauddin M, Phansalkar N, Patki P, Diwanay S, Patwardhan B.
Studies on the immunomodulatory effect of Ashwagandha.
J Ethnopaharmacol 1996;50:69‑76.
Nishiguri C, Yorosh DB, Donawho C, Kripke ML. The immune
system in ultraviolet carcinogenesis. J Invest Dermatol Symp Proc
;1:143‑6.
Lakhashe S, Thakar M, Godbole S, Tripathy S, Paranjape R. HIV
infection in India: Epidemiology, molecular epidemiology and
pathogenesis. J Biosci 2008;33:515‑25.
Ramnath V, Girija K, Kuttan R. Immunomodulatory effect of Abrin
in normal mice. Amala Cancer Bul 2001;21:76‑81.
Patwardhan B, Kalbag D, Patki PS, Nagsampagi BA. Search
of immunomodulatory agents: A review. Indian Drugs
;28:348‑58.
Premanathan M, Rajendran S, Ramanathan T, Kathiresan K,
Nakashima H, Yamamoto N. A survey of some Indian
medicinal plants for anti‑human immunodeficiency virus (HIV)
activity. Indian J Med Res 2000;112:73‑7.
Mullurwar VR, Pathak, AK. Studies on immunomodulatory
activity of Ficus reigiosa. Indian J pharm Edu Res 2008;42:341‑3.
López V, Calvo MI. White tea (Camellia sinensis Kuntze) exerts
neuroprotection against hydrogen peroxide‑ induced toxicity in
PC12 cells. Plant Foods Hum Nutr 2011;66:22‑6.
De Mejia EG, Ramirez‑Mares MV, Puangpraphant S. Bioactive
components of tea: Cancer, inflammation and behavior. Brain
behav Immun 2009;23:721‑31.
Graham HN. Green tea composition, consumption, and
polyphenol chemistry. Prev Med 1992;21:334‑50.
Leung LK, Su Y, Chen R, Zang Z, Huang Y, Chen ZY. Theaflavins in
black and catechins in green tea are equally effective antioxidants.
J Nutr 2001;131:2248‑51.
Kim HJ, Jeon SM, Lee MK, Jung UJ, Shin SK, Choi MS. Antilipogenic
effect of green tea extract in C57BL/6J‑Lep ob/ob mice. Phytother
Res 2009;23:467‑71.
Salminen WF, Yang X, Shi Q, Greenhaw J, Davis K, Ali AA.
Green tea extract can potentiate acetaminophen‑induced
hepatotoxicity in mice. Food Chem Toxicol 2012;50:1439‑46.
International Conference on Harmonization (ICH) of Technical
Requirements for the Registration of Pharmaceuticals
for Human Use, Validation of analytical procedures, ICH‑Q2A,
Geneva; 1995.
McDonald S, Prenzler PD, Autolovich M, Robards K. Phenolic
content and antioxidant activity of olive extracts. Food Chem
;73:73‑84.
Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid
content in propolis by two complementary colorimetric methods.
J Food drug analysis 2002;10:178‑82.
Puri A, Saxena RP, Saxena KC, Srivastava V, Tandon JS.
Immunostimulant activity of Nyctanthes arbour‑tristis L.
J Ethnopharmacol 1994;42:31‑7.
Manjarekar PN, Jolly CI, Narayanan S. Comparative studies of the
immunomodulatory activity of Tinospora cordifolia and Tinospora
sinensis. Fitoterapia 2000;71:254‑7.
Vigila AG, Baskaran X. Immunomodulatory effect of coconut
protein on cyclophosphamide induced immune suppressed Swiss
albino mice. Ethnobot Leaflets 2008;12:1206‑12.
Agarwal R, Diwanay S, Patki P, Patwardhan B. Studies on
immunomodulatory activity of Withania somnifera (Ashwagandha)
extracts in experimental immune inflammation. J Ethnopharmacol
;67:27‑35.
Mitsuoka A, Mitsuo B, Morikawa S. Enhancement of DTH by
depletion of suppressor T‑cells with cyclophosphamide in mice.
Nature 1976;262:77‑9.
Mitra SK, Gupta M, Sarma, DN. Immunomodulatory effect of
VIM‑133. Phytother Res 1999;13:341‑3.
Benacerraf B. A hypothesis to relate the specificity of T lymphocytes
and the activity of I region specific Ir genes in macrophages and
borrower lymphocytes. J Immunol 1978;120:1809‑32.
Makare N, Bodhankar S, Rangari V. Immunomodulatory activity of
alcoholic extract of Magnifera indica L.in mice. J Ethanopharmacol
;78:133‑49.
De La Fuente M, Victor VM. Anti‑oxidants as modulators of
immune function. Immunol Cell Biol 2000;78:49‑54.
Horrigan LA, Kelly JP, Connor TJ. Immunomodulatory effects of
caffeine: Friend or foe. Pharmacol Ther 2006;111:877‑92.
Matsunaga K, Friedman H, Yamamoto Y. Epigallocatechin
Gallate, a Potential Immunomodulatory Agent of Tea
Components, Diminishes Cigarette Smoke Condensate‑Induced
Suppression of Anti‑Legionella pneumophila Activity and
Cytokine Responses of Alveolar Macrophages. Clin Diagn Lab
Immunol 2002;9:864‑71.
