Systemic administration of fractions from Nyctanthes arbor‑tristis attenuates chronic inflammatory response in Freund’s‑complete‑adjuvant‑induced arthritis in rats
DOI:
https://doi.org/10.22377/ijgp.v8i3.376Abstract
Object: The present study was aimed to assess the anti‑arthritic activity of chloroform and n‑butanolic fraction of Nyctanthes
arbor‑tristis leaf extract against Freund’s‑complete‑adjuvant (FCA)‑induced arthritis in rats. Material and Method: Ethanolic extract of Nyctanthes arbor‑tristis leaves were fractionated in various organic solvents and on the basis of results of anti‑inflammatory activity, chloroform and n‑butanolic fractions were selected for anti‑arthritic activity. The active chloroform and n‑butanolic fraction were administered at the dose of 50 and 75 mg/kg body weight. The effects of both fractions on liver alkaline phosphatase (ALP),acid phosphatase (ACP) and lactate dehydrogenase (LDH) levels and malonaldehyde (MDA), glutathione (GSH) and superoxide dismutase (SOD) from articular cartilages in arthritic animals were studied. Prednisolone (10 mg/kg) was used as standard.
Results: In FCA‑induced arthritis, the chloroform and n‑butanolic fraction showed a highly significant reduction in paw volume
(75 mg/kg–76.49%; 70.94%). The levels of various membrane marker enzymes and oxidative free radicals were significantly decreased in the both fraction treated groups and GSH and SOD activities were significantly increased compared with the arthritic control. The chloroform fraction showed most prominent activity as compared to n‑butanolic fraction. Conclusion: The possible mechanism of action of the chloroform fraction of Nyctanthes arbor‑tristis leaf extract may be due to either stabilizing action of membrane marker enzymes or inhibition of oxidative free radicals and thereby preventing the spread of inflammation. Future studies will provide new insights into the anti‑arthritic activity of Nyctanthes arbor‑tristis and isolation of compound and its possible mechanism of action.
Key words: Arthritis, chloroform and n‑butanolic fraction, Nyctanthes arbor‑tristis, membrane marker enzymes, oxidative free radicals
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