In vitro anthelmintic activity of three medicinal plants against Haemonchus contortus
DOI:
https://doi.org/10.22377/ijgp.v3i1.52Abstract
The development of anthelmintic resistance and the high cost of conventional anthelmintic drugs led to the evaluation of medicinal plants as an alternative source of anthelmintics. In the current study, in-vitro experiments were conducted to determine the possible anthelmintic effects of crude aqueous and hydroalcoholic extracts of the leaves of Chenopodium ambrosioides, Lawsonia inermis and seeds of Jatropha curcas, on eggs and adult Haemonchus contortus. Both extracts of C. ambrosioides and J. curcas inhibited the hatching of eggs at a concentration less than or equal to 2 mg/ml, while the effect of L. inermis was not dose-dependent and didnot inhibit the hatching of eggs of H. contortus, significantly, at all tested concentrations. Based on their ED50, the two most potent extracts using egg hatch assay were the hydroalcoholic extract of C. ambrosioides (0.09 mg/ml) and the aqueous extract of J. curcas (0.1 mg/ml) in a decreasing order of potency. With regard to the effect of extracts on the survival of adult parasites, extracts from C. ambrosioides have shown a moderate effect, while J. curcas and L. inermis have shown no statistically significant effect on the survival of adult parasites at the concentrations tested, and the few mortality cases recorded were not dose-dependent (P < 0.05). The overall findings of the present study have shown that C. ambrosioides and J. curcas contain possible anthelmintic compounds
and further evaluation of these plants should be carried out.
Key words: Anthelmintic activity, Chenopodium ambrosioides, Haemonchus contortus, Jatropha curcas, Lawsonia inermis
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References
Waller PJ. Sustainable helminth control of ruminants in developing
countries. Vet Parsitol 1997;71:195-207.
Perry B, Randolph T, Mcdermott J, Sones K, Tornton, PK. Investing
in animal health research to alleviate poverty. Nairobi, Kenya:
ILRI; 2002. p. 148.
Waller PJ, Thamsborg SM. Nematode control in green ruminant
Production systems. Trends Prasitol 2004;20:493-7.
Hammond JA, Fielding D, Bishop SC. Prospects for plant
anthelmintics in tropical veterinary medicine. Vet Res Commun
;21:213-28.
Fajmi AK, Taiwo AA. Herbal remedies in animal parasitic diseases
in Nigeria: A review. Afr J Biotec 2005;4:303-7.
Gathuma JM, Mbaria JM, Wanyama J, Kaburia HF, Mpoke L,
Mwangi JN, et al. Efficacy of Myrsine africana, Albizia anthelmintica
and Hilderbrantia sepalosa herbal remedies in Samburu district,
Kenya. J Ethnopharmcol 2004;91:7-12.
Githiori JB. Evaluation of anthelmintic properties of ethnoveterinary
plant preparations used as livestock dewormers by pastoralists and
smallholder farmers in Kenya. Doctoral Thesis, Swedish University
of Agricultural Sciences, Uppsala, Sweden: 2004.
Iqbal Z, Lateef M, Ashraf M, Jabbar A. Anthelmintic activity of
Artemisia brevifolia in sheep. J Ethopharmacol 2004;93:265-8.
Eguale T, Tilahun G, Debella A, Feleke A, Makkonen E. Haemonchus
contortus: In vitro and in vivo anthelmintic activity of aqueous
and hydroalcoholic extracts of Hedera helix. Exp Parasitol
;116:340-5.
Vollesen K. Euphorbaceae. In: Edwards S, Mesfin T, Hedberg I,
editors. Flora of Ethiopia and Eritrea; Canellaceae to Euphorbaceae.
Vol. 2 Addis Ababa, Ethiopia: 1995. p. 324.
Nwude N, Ibrahim MA. Plants used in traditional veterinary
medicinal practice in Nigeria. J Vet Pharm Ther 1980;3:261-73.
Nsekuye B. Traditional Veterinary Practice in Africa, Technical
cooperation-Federal republic of Germany, Eschaborn; 1994.
Ali BH, Bashir AK, Tanira MO. Anti-inflammatory, antipyretic,
and analgesic effects of Lawsonia inermis L. (henna) in rats.
Pharmacology 1995;51:356-63.
Singh VK, Pandey DK. Fungitoxic studies on bark extract of
Lawsonia inermis against ring worm fungi. Hindustan Antibiot
Bull 1989;31:32-5.
Malekzadeh F. Antimicrobial activity of Lawsonia inermis L. Appl
Microbiol 1968;16:663-4.
Dasgupta T, Rao AR, Yadava PK. Modulatory effect of Henna
leaf (Lawsonia inermis) on drug metabolizing phase I and phase II
enzymes, antioxidant enzymes, lipid peroxidation and chemically
induced skin and fore stomach papillomagenesis in mice. Mol Cell
Biochem 2003;245:11-22.
Makkar HP, Aderibigbe AO, Becker K. Comparative evaluation
of non-toxic and toxic varieties of Jatropha curcas for chemical
composition, digestibility, protein degradability and toxic factors.
Food Chem 1998;62:207-15.
Dessisa D. A preliminary economic evaluation of medicinal plants
in Ethiopia: trade, volume and price In: Medhin Z, Abebe D,
editors. Proceedings of the National Workshop on Biodiversity
Conservation and Sustainable use of Medicinal Plants in Ethiopia.
Addis Ababa, Ethiopia: 28th April-May1st, 1998. 2001. p. 176-88.
Makkar HP, Becker K. Nutritional studies on rats and fish (carp
Cyprinus carpio) fed diets containing unheated and heated Jatropha
curcas meal of a non-toxic provenanace. Plant Foods Hum Nutr
;53:183-92.
Friis I, Gilbert MG. Chenopodiaceae. In: Edwards S, Mesfin T,
Sebsebe D, Hedberg I, editors. Flora of ethiopia and eritrea;
Magnoliaceae to flacourtiaceae. Vol. 2, Published by National
Herbarium of Addis Ababa University and Uppsala University,
Uppsala. Sweden: 2000. p. 277.
Ketzis JK, Taylor A, Bowman DD, Brown DL, Warnick LD,
Erb HN. Chenopodium ambrosioides and its essential oil as treatments
for Haemonchus contortus and mixed adult-nematode infections in
goats. Small Rum Res 2002;44:193-200.
Treating livestock with medicinal plants, Beneficial or toxic?
Chenopodium ambrosioides. Available from: http://www.ansci.
cornell.edu/plants/medicinal/epade.html [last accessed on 2007
Oct 11]
Debella A. Manual for phytochemical screening of medicinal
plants, Ethiopian health and Nutrition Research Institute. Addis
Ababa, Ethiopia: 2002. p. 84.
Coles GC, Bauer C, Borgsteede FH, Geerts S, Klei TR, Taylor MA,
et al. World Association for Advancement of Veterinary
Parasitology (WAAVP) Methods for detection of anthelmintic
resistance in nematodes of veterinary importance. Vet Parasitol
;44:35-43.
Raskin I, Ribnicky DM, Komarnytsky S, Ilic N, Poulev A,
Borisjuk N, et al. Plants and human health in the twenty-first
century. Trends Biotechnol 2002;20:522-31.
Kato S. Effects of the oil of Chenopodium ambrosioides on sheep: efficacy
as an anthelmintic for treatment of gastrointestinal nematodes in
sheep, DVM thesis. NY, USA: Cornell University; 1997.
