Study of extraction and HPTLC - UV method for estimation of caffeine in marketed tea (Camellia sinensis) granules
DOI:
https://doi.org/10.22377/ijgp.v3i1.55Abstract
A simple, precise and accurate high-performance thin-layer chromatographic (HPTLC) method has been established and validated for screening and quantitative estimation of caffeine in different extracts of tea samples (Camellia sinenesis). Separation was performed on silica gel 60 F254 HPTLC plates with ethyl acetate:methanol in the proportion of 27: 3 (v/v), as a mobile phase. The determination was carried out in the ultraviolet (UV) region using the densitometric remission-absorbance mode at 274 nm. Maximum recoveryof caffeine was achieved when extracted with 5% diethyl amine in DM water (v/v). The maximum concentration of caffeine in tea
samples was found to be 2.145%, dry weight basis. Caffeine response was found to be linear over the range of 2-14 μg per zone. Limits of detection and quantitation were found to be 40 and 120 ng/spot, respectively. The HPTLC method was validated in terms of precision, accuracy, sensitivity and robustness. Some rare parameters for the HPTLC method like calculation of flow constant (k) and plate efficiency (N) are included specially.
Key words: Caffeine, Camellia sinensis, HPTLC - UV method, theaceae, tea granules
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References
Caffeine and its modulating effects: Draft review of toxicological
literature. Available from http://ntp.niehs.nih.gov/ntp/htdocs/
Chem_Background/ExSumPdf/Caffeine.pdf. [last cited on 2007
Aug 8].
Frei B, Higdon JV. Antioxidant activity of tea polyphenols in vivo:
Evidence from animal studies. J Nutrition 2003;133:3275S-84S.
Yen GC, Chen HY. Antioxidant activity of various tea extracts
in relation to their antimutagenicity. J Agric Food Chem 1995;
:27-32.
Maeta K, Nomura W, Takatsume Y, Izawa S, Inoue Y. Green
tea polyphenols function as prooxidants to activate oxidativestress-
responsive transcription factors in yeasts. Appl Environ
Microbiol 2007;73:572-80.
Kuriyama S, Shimazu T, Ohmori K, Kikuchi N, Nakaya N,
Nishino Y, Tsubono Y, Tsuji I. Green tea consumption and
mortality due to cardiovascular disease, cancer, and all causes
in Japan: the Ohsaki study. JAMA 2006;296:1255-65.
Larsson SC, Wolk A. Tea consumption and ovarian cancer risk in
a population-based cohort. Arch Internal Med 2005;165:2683-6.
Fadhee ZA, Amran S. Effects of black tea extract on carbon
tetrachloride-induced lipid peroxidation in liver, kidneys, and
testes of rats. Phytother Res 2002;16:28-32.
Keene AT, Anderson LA, Phillipson JD. Investigation of Cinchona
leaf alkaloids by high-performance liquid chromatography. J
Chromatogr 1983;260:123-8.
McCalley DV. Analysis of Cinchona alkaloids by highperformance
liquid chromatography. J Chromatogr 1983;260:
-8.
Baltes W, Petersen H, Degner C. Die remissionsspektralphotometrische
Bestimmung Von Trigonellinund Coffein in Kaffee. Z.
Lebensm. Unters.- Frosch. 1973;152:145-6.
Abourashed EA, Mossa JS. HPTLC determination of caffeine in
stimulant herbal products and power drinks. J Pharm biomed
anal 2004;36:617-20.
Sherma J, Beim M. Determination of caffeine in APC tablets by
densitometry on chemically bonded C18 reversed phase thin
layers. J High Resol Chromatogr 1978;1:309-10.
Reijienga JC, Aben GV, Lemmens AA, Verheggen TP, de
Bruijn CH, Everaerts FM. J Chromatogr 1985;320:245-52.
Dogan HN. Simultaneous spectrophotometric determination of
dipyrone and caffeine in pharmaceutical formulations. Indian
J Pharm Sci 1995;57:224-6.
Hahn-Deinstrop E. Applied Thin-Layer Chromatography: Best
Practice and Avoidance of Mistakes. 2nd ed. Wiley-VCH Verlag
GmbH and Co. KgaA, Weinheim; 2007. p. 4-6.
Kowalska T, Kaczmarski K, Prus W. In: Sherma J, Fried B, editos.
Handbook of Thin-Layer Chromatography. 3rd ed. New York:
Marcel Dekker; 2003. p. 52-6.
Halkina T, Sherma J. Comparative evaluation of the performance
of silica gel TLC plates and irregular and spherical-particle
HPTLC plates. Acta Chromatographica 2006;17:261-71.
Misra H, Mehta BK, Jain DC. Optimization of extraction
conditions and HPTLC-UV method for determination of quinine
in different extracts of Cinchona species bark. Rec Nat Prod
;2:107-15.
Misra, et al.: Study of extraction and HPTLC-UV method for determination of caffeine
