Antidiabetic activity of polyherbomineral formulation: Chandrakala rasa

Abstract

Objective: Chandrakala rasa (CKR), a herbomineral formulation is used to treat diabetes mellitus in ayurvedic system of health care. The present study aims to evaluate the antihyperglycemic potential of CKR in normoglycemic and streptozotocin (STZ)-nicotinamide (NAD)-induced Type 2 diabetic rats. Materials and Methods: Effects of CKR (100, 200 and 400 mg/kg, p.o.) on hypoglycemia as well as on oral glucose tolerance test (OGTT) were evaluated in normoglycemic rats by measuring the blood glucose concentrations. Similarly, blood glucose level was measured after 7, 14 and 21 days in STZ-NAD-induced diabetic rats treated with CKR. Different biochemical parameters such as total cholesterol, triglyceride, low density lipoprotein-cholesterol, and high density lipoprotein-cholesterol were estimated in a blood sample. In vivo antioxidant potential of CKR was measured in isolated liver sample of rats. Results: CKR (400 mg/kg, p.o.) did not show any hypoglycemic effect in normoglycemic rats. In OGTT, it significantly reduced the hike in blood glucose levels within 30-60 min after glucose administration without causing any hypoglycemic effect. Administration of CKR significantly reduced the fasting blood glucose levels on 7th, 14th and 21st days in STZ-NAD-induced diabetic rats. Treatment of rats with CKR reversed plasma lipid profile as well as increases liver glycogen level significantly in STZ-NAD-induced diabetic rats. Treatment with CKR in diabetic rats significantly restored the levels of lipid per-oxidation, superoxide dismutase and catalase as compared to negative control rats. Conclusion: The present study showed that CKR has antidiabetic activity probably because of antioxidant potential