Phytochemical analysis and antibacterial activity of the Hugonia mystax aerial parts

Abstract

Background: The microorganisms, in particular, bacterial strains have been getting resistance to antibiotics due to indiscriminate use of them against different diseases. Hence, there is a need to isolate new antibiotics from natural sources which have less cost, more efficacy, and less side effects. Aim: Hugonia mystax has been using in a traditional medicine. On the basis of the ethno medical, folkloric information the author selected the H. mystax aerial parts to study its phytochemical constituents and capacity of its antibacterial activity to provide the scientific evidence to its traditional use. Materials and Methods: Phytochemical analysis was performed using standard test procedures and antibacterial activity was tested using cup plate method. Results: Qualitative phytochemical screening of H. mystax extracts exposed the presence of different phytochemical constituents in it like steroids, terpenoids, flavonoids, alkaloids, glycosides, phenols, tannins, saponins, and carbohydrates. The extracts gave negative results for the amino acids and oils. The selected plant extracts showed the antibacterial activity along with the standard drug rifampicin. Ethyl acetate extract showed more activity on tested microorganisms compared to hydroalcoholic and hexane extracts. Three extracts showed more antibacterial activity on Gram-negative bacteria than Gram-positive bacteria. Conclusion: The further research is need to evaluate more pharmacological activities and in isolation of bioactive compounds