Standardization protocol development of hydroalcoholic extract of fruits of Citrullus Colocynthis against anti-arthritic activity

Abstract

Aim: So, in the present study, it was targeted to develop standardization protocol of hydroalcoholic extract of fruits of Citrullus colocynthis against the anti-arthritic activity. Materials and Methods: Hydroalcoholic extract of fruit was prepared, and the physical, phytochemical, and chromatographic evaluation of extract were carried out then after pharmacological evaluation for the anti-arthritic activity was done with paw edema method and membrane markers (aspartate transaminase [AST] and alanine transaminase [ALT]). Results and Discussion: The percentage yield of extract was found to be 2.915%. The phytochemical investigations and high performance thin layer chromatography (HPTLC) analysis indicate the presence of Quercetin, a flavonoid which may be responsible for its anti-arthritic activity. Evaluation of paw volume and membrane markers (AST and ALT) showed significant results against anti-arthritic activity Conclusion: The biological activity concluded that C. colocynthis fruit’s hydroalcoholic extract has shown significant anti-arthritic activity which may be due to some sort of phytochemicals such as flavonoids