Augmented oxidative stress in hyperglycemic albino mice exposed to cigarette smoke

Abstract

Aim: Our experiment aimed to evaluate the effects of sidestream cigarette smoke (CS) exposed to diabetic mice. Oxidative stress was evoked by CS and hyperglycemia in albino mice. Materials and Methods: The mice were exposed to CS of two cigarettes for 15 min, twice daily 6 days a week for 16 weeks. After the experimental period, the mice in the different groups were sacrificed by decapitation. Lung tissue was collected in an ice-cold container to carry out biochemical estimation. Oxidative stress was assessed in experimental groups. Results and Discussion: The serum insulin levels were recorded a significant depletion in Groups II, III, and IV when compared to that of control animals. The levels of thiobarbituric acid reactive substances and activity levels of glutathione-s-transferase were significantly increased in experimental groups when compared with control groups. The antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase were significantly depleted in the experimental animals when compared to that of normal animal. Conclusion: Oxidative stress induced by sidestream CS, hyperglycemia, and both in combination aggrandize lipid peroxidation.