Phytoremedial effect of Lens culinaris against doxorubicin-induced nephrotoxicity in male Wistar rats

Abstract

Objectives: The main objective of current study was to determine the phytoremedial effect of ethanol extract of seeds of Lens culinaris against doxorubicin-induced nephrotoxicity using biochemical and histopathological approaches. Materials and Methods: Ethanol extract of seeds of L. culinaris was prepared by hot extraction method, and preliminary phytochemical studies had been carried out. Nephrotoxicity was induced in male Wistar rats by single intraperitoneal administration of doxorubicin (15 mg/kg b.wt.). Nephroprotector effect of the extract was screened at two different dose levels, i.e. 200 and 400 mg/kg b.wt. by oral administration for 8 days. Nephrotoxicity was assessed by determining blood urea nitrogen (BUN), serum creatinine (SC), serum total protein (STP), urinary total protein (UTP), and urinary creatinine (UCr). Renal oxidative stress markers superoxide dismutase (SOD), catalase (CAT), lipid peroxidation (LPO), and glutathione reduced (GSH) were also measured in kidney tissue. Results: The treatment with ethanol extract significantly decreased the levels of BUN, SC, STP, UTP and LPO which were elevated by doxorubicin induction. In addition, extract also ameliorated the levels of UCr, SOD, CAT and GSH in dose-dependent manner. Histopathological studies had also substantiated the biochemical parameters. Conclusion: The results suggest that ethanol extract of seeds of L. culinaris possess potent phytoremedial effect against doxorubicin-induced nephrotoxicity.