Formulation of anti-halitosis mouthwash using aqueous extract of Mangifera indica L. kernel based on the evaluation of its antioxidant, antibacterial, and hemolytic activity

Abstract

Aim: To formulation anti-halitosis mouthwash using aqueous extract of Mangifera indica L. kernel based on the antioxidant, antibacterial, and hemolytic activity of the kernel. Materials and Methods: Methanolic and aqueous extract of M. indica L. kernel were studied for their antioxidant, antimicrobial, and hemolytic properties by hydroxyl free radical scavenging assay, well diffusion method and blood lysis method. Based on the results, mouthwash was formulated from aqueous extract and its antimicrobial and antihalitotic activity was determined by well diffusion method. Results and Discussions: The aqueous extract contains the more protein and free phenols than the methanolic extract. Moreover, the aqueous extract showed the higher hydroxyl scavenging activity than the methanolic extract. In antibacterial assay against selected pathogens, it showed following results. Water extract gave better zones for Bacillus cereus, Neisseria meningitides, Pseudomonas fluorescence, and Bacillus subtilis than methanolic extract. For anti-halitosis activity study, mouth swabs of five patients suffering from halitosis plated on Muller-Hinton agar and inhibition of growth were examined by measuring the zones. The formulated mouthwash showed good results Conclusion: Based on our study, we found that water extract of the kernel possess excellent antioxidant and antibacterial activity, based on which we have formulated and evaluated mouthwash which showed good activity against buccal microflora obtained from halitosis affected patients. Mango kernel can be used as a potent antihalitotic agent in the upcoming days.