In vitro antioxidant activity of ethanolic extract of leaves of cnidoscolus chayamansa Mc Vaugh (Euphorbiaceae)

Abstract

Background: This study was aimed to investigate the antioxidant activities of the ethanolic extract of leaves of Cnidoscolus chayamansa Mc Vaugh (Euphorbiaceae). Materials and Methods: The antioxidant activities of extract have been evaluated using a range of in vitro assays. In these studies, the percentage inhibition values were found to be in 2, 2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO), hydroxyl radical scavenging assays, respectively. Results and Discussion: The results were analyzed statistically by the regression method. Its antioxidant activity was estimated by inhibitory concentration value, and the values are 262.02 μg/ml (DPPH radical scavenging), 343.01 μg/ml (NO scavenging), and 224.47 μg/ml (hydroxyl radical scavenging activity). In all the testing, a significant correlation exists between concentrations of the extract and percentage inhibition of free radicals. The antioxidant property may be related to the polyphenol and flavonoids present in the extract. Conclusion: This study on in vitro antioxidant activities of C. chayamansa, which justifies the ethnomedical use of this plant.